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Sample GSM98282

Query DataSets for GSM98282

Status

Public on Feb 23, 2007

Title

30%sugar_HS_2

Sample type

RNA

Source name

mRNA

Organism

Saccharomyces cerevisiae

Characteristics

Commercial baker's yeast specilized for use in high-sugar dough.

Extracted molecule

polyA RNA

Extraction protocol

Each samples of total RNA were extracted using the hot phenol method. Poly (A)+ RNA was isolated from the total RNA by using an Oligotex® dt30 (super) mRNA purification kit (Takara, Japan) according to the manufacturer’s instructions.

Label

Biotin

Label protocol

Labeling was done according to the Affymetrix GeneChip® Expression Analysis Technical Manual.

Description

After 60min fermentation using 30% sucrose LF medium.

Data processing

Affymetrix GeneChip Operating Software Version 1.2

Submission date

Feb 23, 2006

Last update date

Feb 27, 2006

Contact name

Jun Shima

E-mail(s)

shimaj@nfri.affrc.go.jp

Organization name

National Food Research Institute

Department

Applied Microbiology

Lab

Yeast

Street address

1-2-12 Kannondai

City

Tsukuba

State/province

Ibaraki

ZIP/Postal code

305-8642

Country

Japan

Platform ID

GPL2529

Series (1)

GSE4295

Sugar stress during fermentation (baker's yeast)

Data table header descriptions
ID_REF
VALUE	Normalized value (Per Chip, Per Gene)
BKD_RAW	Control signal (calculated :GeneSpring)
SIGNAL_RAW	Raw intensities (measured: Affymetrix GeneChip)
ABS_CALL	Flag (Affymetrix)

Data table
ID_REF	VALUE	BKD_RAW	SIGNAL_RAW	ABS_CALL
1769308_at	0.988	61.42	60.68	P
1769309_at	0.37	18.01	6.67	A
1769310_at	0.0363	18.01	0.653	A
1769311_at	1.521	1,306	1,987	P
1769312_at	0.507	159.2	80.66	P
1769313_at	0.471	103	48.48	P
1769314_at	1.581	2,577	4,073	P
1769315_at	0.267	18.01	4.801	A
1769316_s_at	0.62	18.01	11.17	A
1769317_at	0.57	624.9	356.4	P
1769318_at	0.0456	18.01	0.821	A
1769319_at	0.893	498.4	444.9	P
1769320_at	1.009	231.4	233.5	P
1769321_at	1.708	332.9	568.6	P
1769322_s_at	0.375	249.2	93.33	P
1769323_at	1.018	489.3	498	P
1769324_at	0.775	277	214.7	P
1769325_at	0.584	32.35	18.9	P
1769326_at	0.0328	18.01	0.591	A
1769327_at	0.39	18.01	7.024	A

Total number of rows: 10928

Table truncated, full table size 331 Kbytes.

Supplementary file	Size	Download	File type/resource
GSM98282.CEL.gz	1.5 Mb	(ftp)(http)	CEL
GSM98282.CHP.gz	58.2 Kb	(ftp)(http)	CHP
Processed data provided as supplementary file