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Sample GSM98274

Query DataSets for GSM98274

Status

Public on Feb 23, 2007

Title

0%sugar_HS_2

Sample type

RNA

Source name

mRNA

Organism

Saccharomyces cerevisiae

Characteristics

Commercial baker's yeast specilized for use in high-sugar dough.

Extracted molecule

polyA RNA

Extraction protocol

Each samples of total RNA were extracted using the hot phenol method. Poly (A)+ RNA was isolated from the total RNA by using an Oligotex® dt30 (super) mRNA purification kit (Takara, Japan) according to the manufacturer’s instructions.

Label

Biotin

Label protocol

Labeling was done according to the Affymetrix GeneChip® Expression Analysis Technical Manual.

Description

After 60min fermentation using 0% sucrose LF medium.

Data processing

Affymetrix GeneChip Operating Software Version 1.2

Submission date

Feb 23, 2006

Last update date

Feb 27, 2006

Contact name

Jun Shima

E-mail(s)

shimaj@nfri.affrc.go.jp

Organization name

National Food Research Institute

Department

Applied Microbiology

Lab

Yeast

Street address

1-2-12 Kannondai

City

Tsukuba

State/province

Ibaraki

ZIP/Postal code

305-8642

Country

Japan

Platform ID

GPL2529

Series (1)

GSE4295

Sugar stress during fermentation (baker's yeast)

Data table header descriptions
ID_REF
VALUE	Normalized value (Per Chip, Per Gene)
BKD_RAW	Control signal (calculated :GeneSpring)
SIGNAL_RAW	Raw intensities (measured: Affymetrix GeneChip)
ABS_CALL	Flag (Affymetrix)

Data table
ID_REF	VALUE	BKD_RAW	SIGNAL_RAW	ABS_CALL
1769308_at	14.77	134.1	1,980	P
1769309_at	0.0392	39.3	1.539	A
1769310_at	0.499	39.3	19.61	A
1769311_at	1.001	2,851	2,854	P
1769312_at	1.014	347.4	352.2	P
1769313_at	3.842	224.7	863.5	P
1769314_at	0.605	5,624	3,401	P
1769315_at	0.0275	39.3	1.081	A
1769316_s_at	0.327	39.3	12.86	A
1769317_at	0.288	1,364	392.4	P
1769318_at	0.104	39.3	4.105	A
1769319_at	1.261	1,088	1,372	P
1769320_at	1.437	505.1	725.7	P
1769321_at	1.445	726.6	1,050	P
1769322_s_at	0.184	543.9	100.3	P
1769323_at	1.056	1,068	1,128	P
1769324_at	1.104	604.6	667.4	P
1769325_at	1.844	70.61	130.2	P
1769326_at	0.0369	39.3	1.448	A
1769327_at	0.0255	39.3	1.003	A

Total number of rows: 10928

Table truncated, full table size 328 Kbytes.

Supplementary file	Size	Download	File type/resource
GSM98274.CEL.gz	1.6 Mb	(ftp)(http)	CEL
GSM98274.CHP.gz	57.1 Kb	(ftp)(http)	CHP
Processed data provided as supplementary file