|
| Status |
Public on Feb 23, 2007 |
| Title |
40%sugar_LS_1 |
| Sample type |
RNA |
| |
|
| Source name |
mRNA
|
| Organism |
Saccharomyces cerevisiae |
| Characteristics |
Commercial baker's yeast specilized for use in unsugared dough.
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
Each samples of total RNA were extracted using the hot phenol method. Poly (A)+ RNA was isolated from the total RNA by using an Oligotex® dt30 (super) mRNA purification kit (Takara, Japan) according to the manufacturer’s instructions.
|
| Label |
Biotin
|
| Label protocol |
Labeling was done according to the Affymetrix GeneChip® Expression Analysis Technical Manual.
|
| |
|
| Description |
After 60min fermentation using 40% sucrose LF medium.
|
| Data processing |
Affymetrix GeneChip Operating Software Version 1.2
|
| |
|
| Submission date |
Feb 23, 2006 |
| Last update date |
Feb 27, 2006 |
| Contact name |
Jun Shima |
| E-mail(s) |
shimaj@nfri.affrc.go.jp
|
| Organization name |
National Food Research Institute
|
| Department |
Applied Microbiology
|
| Lab |
Yeast
|
| Street address |
1-2-12 Kannondai
|
| City |
Tsukuba |
| State/province |
Ibaraki |
| ZIP/Postal code |
305-8642 |
| Country |
Japan |
| |
|
| Platform ID |
GPL2529 |
| Series (1) |
| GSE4295 |
Sugar stress during fermentation (baker's yeast) |
|
