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Sample GSM98264 Query DataSets for GSM98264
Status Public on Feb 23, 2007
Title 10%sugar_LS_2
Sample type RNA
 
Source name mRNA
Organism Saccharomyces cerevisiae
Characteristics Commercial baker's yeast specilized for use in unsugared dough.
Extracted molecule polyA RNA
Extraction protocol Each time samples of total RNA were extracted using the hot phenol method. Poly (A)+ RNA was isolated from the total RNA by using an Oligotex® dt30 (super) mRNA purification kit (Takara, Japan) according to the manufacturer’s instructions.
Label Biotin
Label protocol Labeling was done according to the Affymetrix GeneChip® Expression Analysis Technical Manual.
 
Description After 60min fermentation using 10% sucrose LF medium.
Data processing Affymetrix GeneChip Operating Software Version 1.2
 
Submission date Feb 23, 2006
Last update date Feb 27, 2006
Contact name Jun Shima
E-mail(s) shimaj@nfri.affrc.go.jp
Organization name National Food Research Institute
Department Applied Microbiology
Lab Yeast
Street address 1-2-12 Kannondai
City Tsukuba
State/province Ibaraki
ZIP/Postal code 305-8642
Country Japan
 
Platform ID GPL2529
Series (1)
GSE4295 Sugar stress during fermentation (baker's yeast)

Data table header descriptions
ID_REF
VALUE Normalized value (Per Chip, Per Gene)
BKD_RAW Control signal (calculated :GeneSpring)
SIGNAL_RAW Raw intensities (measured: Affymetrix GeneChip)
ABS_CALL Flag (Affymetrix)

Data table
ID_REF VALUE BKD_RAW SIGNAL_RAW ABS_CALL
1769308_at 0.644 0.661 0.426 A
1769309_at 37.59 0.194 7.288 A
1769310_at 25.26 0.194 4.899 A
1769311_at 0.968 14.07 13.62 A
1769312_at 5.666 1.714 9.711 A
1769313_at 7.497 1.109 8.313 A
1769314_at 0.131 27.75 3.637 A
1769315_at 25.76 0.194 4.995 A
1769316_s_at 1.984 0.194 0.385 A
1769317_at 0.257 6.728 1.73 A
1769318_at 1.689 0.194 0.328 A
1769319_at 1.86 5.367 9.983 P
1769320_at 3.088 2.492 7.695 A
1769321_at 1.995 3.585 7.15 A
1769322_s_at 114 2.683 305.8 P
1769323_at 0.535 5.269 2.818 A
1769324_at 0.684 2.983 2.041 A
1769325_at 23.22 0.348 8.09 A
1769326_at 13.22 0.194 2.563 A
1769327_at 2.94 0.194 0.57 A

Total number of rows: 10928

Table truncated, full table size 330 Kbytes.




Supplementary file Size Download File type/resource
GSM98264.CEL.gz 1.3 Mb (ftp)(http) CEL
GSM98264.CHP.gz 62.7 Kb (ftp)(http) CHP
Processed data provided as supplementary file

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