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Sample GSM98261

Query DataSets for GSM98261

Status

Public on Feb 23, 2007

Title

5%sugar_LS_1

Sample type

RNA

Source name

mRNA

Organism

Saccharomyces cerevisiae

Characteristics

Commercial baker's yeast specilized for use in unsugared dough.

Extracted molecule

polyA RNA

Extraction protocol

Each time samples of total RNA were extracted using the hot phenol method. Poly (A)+ RNA was isolated from the total RNA by using an Oligotex® dt30 (super) mRNA purification kit (Takara, Japan) according to the manufacturer’s instructions.

Label

Biotin

Label protocol

Labeling was done according to the Affymetrix GeneChip® Expression Analysis Technical Manual.

Description

After 60min fermentation using 5% sucrose LF medium.

Data processing

Affymetrix GeneChip Operating Software Version 1.2

Submission date

Feb 23, 2006

Last update date

Feb 27, 2006

Contact name

Jun Shima

E-mail(s)

shimaj@nfri.affrc.go.jp

Organization name

National Food Research Institute

Department

Applied Microbiology

Lab

Yeast

Street address

1-2-12 Kannondai

City

Tsukuba

State/province

Ibaraki

ZIP/Postal code

305-8642

Country

Japan

Platform ID

GPL2529

Series (1)

GSE4295

Sugar stress during fermentation (baker's yeast)

Data table header descriptions
ID_REF
VALUE	Normalized value (Per Chip, Per Gene)
BKD_RAW	Control signal (calculated :GeneSpring)
SIGNAL_RAW	Raw intensities (measured: Affymetrix GeneChip)
ABS_CALL	Flag (Affymetrix)

Data table
ID_REF	VALUE	BKD_RAW	SIGNAL_RAW	ABS_CALL
1769308_at	0.78	25.06	19.56	A
1769309_at	1.037	7.347	7.618	A
1769310_at	2.486	7.347	18.27	A
1769311_at	2.91	533	1,551	P
1769312_at	1.244	64.94	80.82	P
1769313_at	0.866	42.02	36.37	P
1769314_at	1.595	1,051	1,677	P
1769315_at	2.197	7.347	16.14	A
1769316_s_at	3.029	7.347	22.25	A
1769317_at	1.478	255	376.7	P
1769318_at	0.145	7.347	1.067	A
1769319_at	1.269	203.4	258.1	P
1769320_at	1.523	94.43	143.8	P
1769321_at	0.906	135.8	123	P
1769322_s_at	1.53	101.7	155.6	P
1769323_at	0.945	199.7	188.7	P
1769324_at	0.645	113	72.95	P
1769325_at	0.625	13.2	8.251	A
1769326_at	1.652	7.347	12.14	A
1769327_at	0.277	7.347	2.032	A

Total number of rows: 10928

Table truncated, full table size 329 Kbytes.

Supplementary file	Size	Download	File type/resource
GSM98261.CEL.gz	1.6 Mb	(ftp)(http)	CEL
GSM98261.CHP.gz	59.0 Kb	(ftp)(http)	CHP
Processed data provided as supplementary file