|
Status |
Public on Jul 23, 2013 |
Title |
CD8+ T cells, baseline, biological replicate 2 |
Sample type |
RNA |
|
|
Source name |
CD8+ T cells, baseline
|
Organism |
Homo sapiens |
Characteristics |
diagnosis: sarcoidosis cell type: CD8+ T cells
|
Treatment protocol |
Participants received the oral CLEAR regimen: Levofloxacin 750 mg on day 1, then 500 mg daily, Ethambutol 1200 mg daily, Azithromycin 500 mg on day 1, then 250 mg daily, and Rifampin 300 mg daily, or a comparative placebo regimen, for a total of 8 weeks, with a 180 day follow-up.
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA extracted using miRNeasy mini kit (Qiagen) according to the manufacturer's instructions.
|
Label |
Cy3
|
Label protocol |
Cy3 labeled RNA was prepared according to the standard Agilent protocol from 50ng total RNA
|
|
|
Hybridization protocol |
Labeled RNA was hybridized for 17 hr at 65C on Agilent 8X60K microRNA array. Arrays were washed according to the manufacturer's protocol.
|
Scan protocol |
Slides were scanned immediately after washing on the Agilent DNA Microarray Scanner (G2505B) using one color scan setting for 8x60K array slides
|
Description |
C21-8_510
|
Data processing |
The scanned images were analyzed with Feature Extraction Software 10.7.3.1 (Agilent) using default parameters.
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|
|
Submission date |
Jul 24, 2012 |
Last update date |
Jul 23, 2013 |
Contact name |
Kusum Vijay Pandit |
E-mail(s) |
panditkusum@yahoo.com
|
Phone |
412-624-9046
|
Organization name |
University of Pittsburgh
|
Department |
Medicine-PACCM
|
Street address |
200, Lothrop street, W1200-25A Biomedical Science Tower
|
City |
Pittsburgh |
State/province |
PA |
ZIP/Postal code |
15213 |
Country |
USA |
|
|
Platform ID |
GPL14550 |
Series (1) |
GSE39606 |
Gene expression data in CD4+ and CD8+ cells in sarcoidosis patients following antimycobacterial therapy |
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