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Sample GSM967928 Query DataSets for GSM967928
Status Public on Jan 20, 2015
Title Brain-Early-onset Alzheimer disease- 4
Sample type RNA
Source name Frozen brain tissue: posterior cingulate at thalamus level
Organism Homo sapiens
Characteristics gender: Male
age at death: 69
post-mortem interval (hours): 3:30
apoe genotype: 3/3
psen1 mutation: None
neuropathological data (braak stage): A3, B3, C3 + CAA moderate
Extracted molecule total RNA
Extraction protocol Total RNA was extracted from frozen brain tissue (posterior cingulate area) using the RNeasy Lipid Tissue Mini Kit (Qiagen)
Label Biotin
Label protocol Labeled ssDNA was prepared according to the standard Affymetrix Terminal labeling Kit protocol.
Hybridization protocol Following fragmentation, 5 ug of ssDNA were hybridized for 16 hr at 45C following Affymetrix standard procedures
Scan protocol GeneChips were scanned according to the standard Affymetrix protocol (Affymetrix Genechip Command Console program, scanner PlateScanner M10)
Description Gene expression data of final stages of Alzheimer's disease
Data processing Raw data was background adjusted, normalized and summarized by Robust Multi-array Analysis (RMA) using the Affy package (version 1.30.0) from Bioconductor on R language (version 2.13.2).
Submission date Jul 17, 2012
Last update date Jan 20, 2015
Contact name Anna Antonell
Organization name Hospital Clínic Barcelona
Department Unitat d'Alzheimer i altres transtorns cognitius
Lab Malalties neurodegeneratives
Street address C/Casanova, 143
City Barcelona
ZIP/Postal code 08036
Country Spain
Platform ID GPL11532
Series (1)
GSE39420 Gene expression profile of sporadic and PSEN1 early-onset Alzheimer’s Disease

Data table header descriptions
VALUE log2 RMA normalized signal intensity

Data table
7892501 2.460
7892502 4.572
7892503 2.593
7892504 8.604
7892505 2.308
7892506 3.054
7892507 5.070
7892508 6.722
7892509 10.378
7892510 4.690
7892511 4.280
7892512 5.162
7892513 2.935
7892514 9.750
7892515 9.264
7892516 2.929
7892517 6.873
7892518 3.335
7892519 3.831
7892520 8.148

Total number of rows: 33297

Table truncated, full table size 456 Kbytes.

Supplementary file Size Download File type/resource
GSM967928_E4.CEL.gz 4.6 Mb (ftp)(http) CEL
Processed data included within Sample table

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