|
| Status |
Public on Jul 17, 2012 |
| Title |
7603, left breast tumor |
| Sample type |
RNA |
| |
|
| Source name |
7603, left breast tumor
|
| Organism |
Homo sapiens |
| Characteristics |
kras: WT
fgfr1: Amplified
age: 60
tumor type: Breast
Stage: I
treatment: Letrozole; 16 days
|
| Treatment protocol |
The patient was treated with 16 days of letrozole leading up to definitive breast surgery of both the left and right bilateral tumors
|
| Growth protocol |
Surgical tumor specimens from ER+ breast cancer patients were utilized for RNA extraction and hybridization to Affymetrix Gene expression microarrays
|
| Extracted molecule |
total RNA |
| Extraction protocol |
TriZOL followed by RNEasy extraction
|
| Label |
biotin
|
| Label protocol |
Reactions were run with 100ng of Total RNA used per reaction in the Affymetrix IVT-Express Kit (Part #901225, Lot#0901008, Exp 040110) on 030310. The reactions were run through First and Second Strand synthesis, followed by In Vitro Transcription (IVT) using a 16 hour incubation to generate aRNA.
|
| |
|
| Hybridization protocol |
7.5ug of samples were fragmented and 6ug was put into hyb cocktail for use with the Gene Titan
|
| Scan protocol |
Samples were hybridized and scanned using the Gene Titan Affyemtrix instrument according to manufacturer's protocols
|
| Description |
RMA normalized gene expression data from frozen surgical tumor
|
| Data processing |
Data were normalized by RMA in R using the Affy package
|
| |
|
| Submission date |
Jul 16, 2012 |
| Last update date |
Jul 17, 2012 |
| Contact name |
Justin M Balko |
| Organization name |
Vanderbilt University
|
| Department |
Medicine
|
| Lab |
Arteaga
|
| Street address |
777 Preston Research Building
|
| City |
Nashville |
| State/province |
TN |
| ZIP/Postal code |
37232-6307 |
| Country |
USA |
| |
|
| Platform ID |
GPL13158 |
| Series (1) |
| GSE39387 |
Discordant cellular response to pre-surgical letrozole in bilateral synchronous ER+ breast cancers with a KRAS mutation or FGFR1 gene amplification |
|
