|
| Status |
Public on Sep 01, 2012 |
| Title |
obese_liver_ER_20080725 |
| Sample type |
RNA |
| |
|
| Source name |
obese_liver_ER
|
| Organism |
Mus musculus |
| Characteristics |
Sex: male
age: 3 months old
tissue: liver
rna subtype: Endoplasmic Reticulum-associated polysome mRNA
strain: C57BL/6
genotype: ob/ob
diet: overnight fasted, Diet 5058
|
| Treatment protocol |
with or without overnight fasting from 5pm-10am
|
| Growth protocol |
12-hour day/12-hour night cycle, Diet 5058 from Lab Diet, 3 months of age
|
| Extracted molecule |
total RNA |
| Extraction protocol |
ER fractionation and Trizol LS extraction
|
| Label |
biotin
|
| Label protocol |
GeneChip Expression 3’-Amplification Reagents One-Cycle cDNA Synthesis system (P/N 900431)
|
| |
|
| Hybridization protocol |
Affymetrix GeneChip® Manual (rev. 1.3)
|
| Scan protocol |
Affymetrix GeneChip® Manual (rev. 1.3)
|
| Description |
male, 3 months old, ob/ob in C57BL/6 background, overnight fasted, Diet 5058
|
| Data processing |
Affymetrix GeneChip Operating Software , MAS5.0, R
|
| |
|
| Submission date |
Jul 16, 2012 |
| Last update date |
Sep 01, 2012 |
| Contact name |
Suneng Fu |
| E-mail(s) |
sunengfu@hsph.harvard.edu
|
| Phone |
617-432-1951
|
| Fax |
617-432-1826
|
| Organization name |
Harvard School of Public Health
|
| Department |
Genetics and Complex Diseases
|
| Lab |
Gokhan S. Hotamisligil
|
| Street address |
665 Huntington Ave
|
| City |
Bostn |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE39375 |
Polysome profiling in liver identifies dynamic regulation of endoplasmic reticulum translatome by obesity and fasting |
|
