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Sample GSM9516 Query DataSets for GSM9516
Status Public on Oct 29, 2003
Title 0 hr Ctl A-chip 6-11-02
Sample type RNA
Source name IB3-1 bronchial epithelial cell line
Organism Homo sapiens
Extracted molecule total RNA
Description IB3-1 (deltaF508/W1282X) bronchial epithelial cells were cultured in T75 flasks with gentamicin-free LHC-8 medium. A T75 flask of confluent IB3-1 cells was rinsed twice with ice cold Hank’s buffer then scraped into 3ml of ice cold TRIzol (Gibco BRL) then rinsed with 3 ml ice cold TRIzol and the mRNA was isolated according to the TRIzol protocol. Cells were fed with 10 ml of media every 2 to 3 days. Array images were assessed by eye to confirm scanner alignment and the absence of significant bubbles or scratches. Chip scanning and expression analysis was performed using Microarray Analysis Suite 5.0 software and scaled to an average probe set intensity of 150.
Submission date Sep 03, 2003
Last update date Mar 21, 2011
Contact name Jerry M. Wright
Phone 410-614-5010
Organization name Johns Hopkins Medical Institutions
Department Physiology
Street address 725 N. Wolfe St
City Baltimore
State/province MD
ZIP/Postal code 21205
Country USA
Platform ID GPL96
Series (1)
GSE620 Gene expression profile analysis of 4-phenylbutyrate treatment of IB3-1 bronchial epithelial cell line
Reanalyzed by GSM694164

Data table header descriptions
VALUE chip scaled to 150
ABS_CALL Present/Marginal/Absent

Data table
AFFX-BioB-5_at 156.5 P
AFFX-BioB-M_at 403.5 P
AFFX-BioB-3_at 185.2 P
AFFX-BioC-5_at 504.2 P
AFFX-BioC-3_at 417.4 P
AFFX-BioDn-5_at 386.3 P
AFFX-BioDn-3_at 2892.6 P
AFFX-CreX-5_at 4439.9 P
AFFX-CreX-3_at 6681.1 P
AFFX-DapX-5_at 5.3 A
AFFX-DapX-M_at 21.3 A
AFFX-DapX-3_at 8.2 A
AFFX-LysX-5_at 5.2 A
AFFX-LysX-M_at 3.6 A
AFFX-LysX-3_at 12.8 A
AFFX-PheX-5_at 1.4 A
AFFX-PheX-M_at 2.2 A
AFFX-PheX-3_at 18.2 A
AFFX-ThrX-5_at 4.0 A
AFFX-ThrX-M_at 13.8 A

Total number of rows: 22283

Table truncated, full table size 394 Kbytes.

Supplementary file Size Download File type/resource
GSM9516.CEL.gz 3.5 Mb (ftp)(http) CEL

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