|
| Status |
Public on Jan 11, 2014 |
| Title |
GFP_Day5, biological rep2 |
| Sample type |
RNA |
| |
|
| Source name |
MEFs infected with retroviruses encoding GFP, day 5
|
| Organism |
Mus musculus |
| Characteristics |
cell type: mouse embryonic fibroblasts (MEFs)
retrovirus: GFP
time: day 5
|
| Treatment protocol |
MEFs were infected with retroviruses encoding the four transcription factors (Oct4, Sox2, Klf4 and c-Myc), three transcription factors (Oct4, Sox2 and Klf4) or GFP (control) at day 0.
|
| Growth protocol |
MEFs were maintained in Dulbecco's Modified Eagle's medium (DMEM) containing 10% foetal bovine serum (FBS).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNAs were extracted using the Qiagen RNeasy kit according to the manufacturer's protocol. On-column DNase digestion was performed.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNAs were prepared from 120 ng (rep1) or 300 ng (the others) total RNAs according to the Affymetrix Whole Transcript (WT) Sense Target Labeling Assay manual.
|
| |
|
| Hybridization protocol |
Standard Affymetrix protocol
|
| Scan protocol |
Standard Affymetrix protocol with Affymetrix Scanner 3000 7G
|
| Description |
GFP_Day5-2
Gene expression data from MEFs infected with retroviruses encoding GFP, day 5.
|
| Data processing |
The probeset signals were calculated using Agilent GeneSpring software.
|
| |
|
| Submission date |
Jun 05, 2012 |
| Last update date |
Sep 08, 2016 |
| Contact name |
Miki Ebisuya |
| E-mail(s) |
miki.ebisuya@embl.es
|
| Organization name |
EMBL
|
| Department |
EMBL Barcelona
|
| Lab |
Lab for Synthetic Developmental Biology
|
| Street address |
PRBB Building, C/ Dr. Aiguader, 88
|
| City |
Barcelona |
| ZIP/Postal code |
08003 |
| Country |
Spain |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE38509 |
Gene expression profiling in the reprogramming process |
|
