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Sample GSM9432

Query DataSets for GSM9432

Status

Public on Sep 03, 2003

Title

13-4-aCy5

Sample type

RNA

Channel 1

Source name

segregant from BY4716 x wild wine strain RM11-1a

Organism

Saccharomyces cerevisiae

Extracted molecule

total RNA

Channel 2

Source name

reference pool of BY4716 strain

Organism

Saccharomyces cerevisiae

Extracted molecule

total RNA

Description

RNA from a haploid F1 segregant from a cross between the
lab strain BY4716 (isogenic to S288c) and haploid derivative RM11-1a from a wild isolate
strain from a California vineyard collected by R.
Mortimer. The reference sample is RNA from haploid
BY4716. See Yvert et al. Nature Genetics 2003.
NOTE: REFERENCE SAMPLE IS DIFFERENT FROM THE ONE USED
IN Brem et al. Science 2002 296:752-755. It corresponds
to the same strain but comes from an independent culture.
Lot batch =

Submission date

Sep 02, 2003

Last update date

May 28, 2005

Contact name

Gael Yvert

E-mail(s)

yvert@insa-tlse.fr

Phone

011-33-5-61-55-94-25

Organization name

Fred Hutchinson Cancer Research Center

Lab

Kruglyak

Street address

City

Seattle

State/province

ZIP/Postal code

98109

Country

USA

Platform ID

GPL118

Series (1)

GSE617

Trans-acting regulatory variation in Saccharomyces cerevisiae

Data table header descriptions
ID_REF	spot number from platform GPL118 (yeast cDNA arrays manufactured
CH1_MEAN	median signal at 635 nm
CH1_BKD_MEAN	median background signal at 635 nm
CH2_MEAN	median signal at 532 nm
CH2_BKD_MEAN	median background signal at 532 nm
VALUE	First compute l = log2 (median 635 - median background 635) - log2 (median 532 - median background 532). Do this for every spot on chip c. Next compute m, the mean of these l values over all spots on chip c. Now subtract m from every l value on chip c to get the final normalized log ratio n. If the SAMPLE has Cy3 in the name, n is the VALUE here; if the SAMPLE has Cy5 in the name, -n is the VALUE.
FLAG	GenePix flag; all spots with flag < 0 (defective spot or data) removed

Data table
ID_REF	CH1_MEAN	CH1_BKD_MEAN	CH2_MEAN	CH2_BKD_MEAN	VALUE	FLAG
0	1632	42	1375	53	0.237351	0
1	725	51	862	58	-0.283401	0
2	708	56	824	67	-0.244375	0
3	943	41	996	50	-0.097666	0
4	391	41	526	50	-0.472560	0
5	993	51	1019	61	-0.053252	0
6	50	50	64	59		-50
7	1328	42	1238	51	0.086617	0
8	1170	42	1434	51	-0.322988	0
9	730	42	868	50	-0.278646	0
10	1015	47	1169	54	-0.232919	0
11	7431	47	6889	56	0.082930	0
12	7949	49	5423	57	0.529052	0
13	2319	47	2067	55	0.146379	0
14	651	45	772	53	-0.275628	0
15	3105	45	3340	53	-0.132194	0
16	3218	48	3472	57	-0.136356	0
17	3006	52	2679	61	0.145251	0
18	923	50	1016	59	-0.161491	0
19	3284	48	3133	57	0.044202	0

Total number of rows: 7085

Table truncated, full table size 208 Kbytes.

Supplementary data files not provided