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Sample GSM9410

Query DataSets for GSM9410

Status

Public on Sep 03, 2003

Title

10-2-dCy5

Sample type

RNA

Channel 1

Source name

segregant from BY4716 x wild wine strain RM11-1a

Organism

Saccharomyces cerevisiae

Extracted molecule

total RNA

Channel 2

Source name

reference pool of BY4716 strain

Organism

Saccharomyces cerevisiae

Extracted molecule

total RNA

Description

RNA from a haploid F1 segregant from a cross between the
lab strain BY4716 (isogenic to S288c) and haploid derivative RM11-1a from a wild isolate
strain from a California vineyard collected by R.
Mortimer. The reference sample is RNA from haploid
BY4716. See Yvert et al. Nature Genetics 2003.
NOTE: REFERENCE SAMPLE IS DIFFERENT FROM THE ONE USED
IN Brem et al. Science 2002 296:752-755. It corresponds
to the same strain but comes from an independent culture.
Lot batch =

Submission date

Sep 02, 2003

Last update date

May 28, 2005

Contact name

Gael Yvert

E-mail(s)

yvert@insa-tlse.fr

Phone

011-33-5-61-55-94-25

Organization name

Fred Hutchinson Cancer Research Center

Lab

Kruglyak

Street address

City

Seattle

State/province

ZIP/Postal code

98109

Country

USA

Platform ID

GPL118

Series (1)

GSE617

Trans-acting regulatory variation in Saccharomyces cerevisiae

Data table header descriptions
ID_REF	spot number from platform GPL118 (yeast cDNA arrays manufactured
CH1_MEAN	median signal at 635 nm
CH1_BKD_MEAN	median background signal at 635 nm
CH2_MEAN	median signal at 532 nm
CH2_BKD_MEAN	median background signal at 532 nm
VALUE	First compute l = log2 (median 635 - median background 635) - log2 (median 532 - median background 532). Do this for every spot on chip c. Next compute m, the mean of these l values over all spots on chip c. Now subtract m from every l value on chip c to get the final normalized log ratio n. If the SAMPLE has Cy3 in the name, n is the VALUE here; if the SAMPLE has Cy5 in the name, -n is the VALUE.
FLAG	GenePix flag; all spots with flag < 0 (defective spot or data) removed

Data table
ID_REF	CH1_MEAN	CH1_BKD_MEAN	CH2_MEAN	CH2_BKD_MEAN	VALUE	FLAG
0	2412	78	1743	70	0.104264	0
1	1179	76	888	68	0.051634	0
2	1055	75	743	69	0.163930	0
3	1210	80	1056	70	-0.179440	0
4	621	99	579	79	-0.313981	0
5	1626	112	1057	89	0.269183	0
6	1827	91	1184	78	0.274313	0
7	2568	76	1669	69	0.263129	0
8	2468	78	1782	70	0.105225	0
9	1261	88	1093	79	-0.165957	0
10	2056	111	1627	98	-0.028921	0
11	11083	145	8356	120	0.033230	0
12	11166	147	7807	123	0.143961	0
13	3996	108	2813	94	0.139849	0
14	1288	109	1143	94	-0.207554	0
15	6446	142	4528	116	0.138732	0
16	7792	162	5594	130	0.105623	0
17	5143	157	3605	127	0.143522	0
18	2634	126	1657	100	0.311666	0
19	5800	115	3895	97	0.205818	0

Total number of rows: 7085

Table truncated, full table size 211 Kbytes.

Supplementary data files not provided