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Sample GSM9387

Query DataSets for GSM9387

Status

Public on Sep 03, 2003

Title

8-5-bCy3

Sample type

RNA

Channel 1

Source name

reference pool of BY4716 strain

Organism

Saccharomyces cerevisiae

Extracted molecule

total RNA

Channel 2

Source name

segregant from BY4716 x wild wine strain RM11-1a

Organism

Saccharomyces cerevisiae

Extracted molecule

total RNA

Description

RNA from a haploid F1 segregant from a cross between the
lab strain BY4716 (isogenic to S288c) and haploid derivative RM11-1a from a wild isolate
strain from a California vineyard collected by R.
Mortimer. The reference sample is RNA from haploid
BY4716. See Yvert et al. Nature Genetics 2003.
NOTE: REFERENCE SAMPLE IS DIFFERENT FROM THE ONE USED
IN Brem et al. Science 2002 296:752-755. It corresponds
to the same strain but comes from an independent culture.
Lot batch =

Submission date

Sep 02, 2003

Last update date

May 28, 2005

Contact name

Gael Yvert

E-mail(s)

yvert@insa-tlse.fr

Phone

011-33-5-61-55-94-25

Organization name

Fred Hutchinson Cancer Research Center

Lab

Kruglyak

Street address

City

Seattle

State/province

ZIP/Postal code

98109

Country

USA

Platform ID

GPL118

Series (1)

GSE617

Trans-acting regulatory variation in Saccharomyces cerevisiae

Data table header descriptions
ID_REF	spot number from platform GPL118 (yeast cDNA arrays manufactured
CH1_MEAN	median signal at 635 nm
CH1_BKD_MEAN	median background signal at 635 nm
CH2_MEAN	median signal at 532 nm
CH2_BKD_MEAN	median background signal at 532 nm
VALUE	First compute l = log2 (median 635 - median background 635) - log2 (median 532 - median background 532). Do this for every spot on chip c. Next compute m, the mean of these l values over all spots on chip c. Now subtract m from every l value on chip c to get the final normalized log ratio n. If the SAMPLE has Cy3 in the name, n is the VALUE here; if the SAMPLE has Cy5 in the name, -n is the VALUE.
FLAG	GenePix flag; all spots with flag < 0 (defective spot or data) removed

Data table
ID_REF	CH1_MEAN	CH1_BKD_MEAN	CH2_MEAN	CH2_BKD_MEAN	VALUE	FLAG
0	90	49	123	63	0.763902	0
1	113	47	146	62	0.562487	0
2	172	47	231	62	0.649659	0
3	168	47	231	62	0.696580	0
4	112	48	140	62	0.499966	0
5	197	51	207	65	0.174486	0
6	145	50	182	65	0.515073	0
7	305	55	386	64	0.579696	0
8	367	52	327	63	-0.040250	0
9	210	52	264	65	0.547408	0
10	360	51	408	64	0.369366	0
11	2860	52	2668	65	0.105196	0
12	3134	55	4494	69	0.737779	0
13	1012	56	1136	72	0.368980	0
14	429	58	476	73	0.333925	0
15	1987	58	1745	73	0.008286	0
16	2568	62	2531	76	0.184900	0
17	1817	63	1780	77	0.171994	0
18	965	64	746	77	-0.214957	0
19	3487	64	3975	74	0.403147	0

Total number of rows: 7085

Table truncated, full table size 210 Kbytes.

Supplementary data files not provided