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Sample GSM9381

Query DataSets for GSM9381

Status

Public on Sep 03, 2003

Title

8-2-dCy3

Sample type

RNA

Channel 1

Source name

reference pool of BY4716 strain

Organism

Saccharomyces cerevisiae

Extracted molecule

total RNA

Channel 2

Source name

segregant from BY4716 x wild wine strain RM11-1a

Organism

Saccharomyces cerevisiae

Extracted molecule

total RNA

Description

RNA from a haploid F1 segregant from a cross between the
lab strain BY4716 (isogenic to S288c) and haploid derivative RM11-1a from a wild isolate
strain from a California vineyard collected by R.
Mortimer. The reference sample is RNA from haploid
BY4716. See Yvert et al. Nature Genetics 2003.
NOTE: REFERENCE SAMPLE IS DIFFERENT FROM THE ONE USED
IN Brem et al. Science 2002 296:752-755. It corresponds
to the same strain but comes from an independent culture.
Lot batch =

Submission date

Sep 02, 2003

Last update date

May 28, 2005

Contact name

Gael Yvert

E-mail(s)

yvert@insa-tlse.fr

Phone

011-33-5-61-55-94-25

Organization name

Fred Hutchinson Cancer Research Center

Lab

Kruglyak

Street address

City

Seattle

State/province

ZIP/Postal code

98109

Country

USA

Platform ID

GPL118

Series (1)

GSE617

Trans-acting regulatory variation in Saccharomyces cerevisiae

Data table header descriptions
ID_REF	spot number from platform GPL118 (yeast cDNA arrays manufactured
CH1_MEAN	median signal at 635 nm
CH1_BKD_MEAN	median background signal at 635 nm
CH2_MEAN	median signal at 532 nm
CH2_BKD_MEAN	median background signal at 532 nm
VALUE	First compute l = log2 (median 635 - median background 635) - log2 (median 532 - median background 532). Do this for every spot on chip c. Next compute m, the mean of these l values over all spots on chip c. Now subtract m from every l value on chip c to get the final normalized log ratio n. If the SAMPLE has Cy3 in the name, n is the VALUE here; if the SAMPLE has Cy5 in the name, -n is the VALUE.
FLAG	GenePix flag; all spots with flag < 0 (defective spot or data) removed

Data table
ID_REF	CH1_MEAN	CH1_BKD_MEAN	CH2_MEAN	CH2_BKD_MEAN	VALUE	FLAG
0	1320	43	1666	62	0.439501	0
1	751	43	1043	63	0.579617	0
2	711	42	645	60	-0.082985	0
3	986	48	1129	67	0.289709	0
4	632	49	714	68	0.258623	0
5	1066	59	1023	79	0.017380	0
6	181	50	186	66		-50
7	1295	43	1959	61	0.710830	0
8	1420	45	1651	64	0.317456	0
9	921	47	924	66	0.083929	0
10	1157	52	1368	71	0.341717	0
11	7084	58	9990	78	0.607058	0
12	7956	67	5403	86	-0.458645	0
13	2431	67	3114	85	0.468197	0
14	966	64	1042	81	0.201994	0
15	3830	63	4937	82	0.476640	0
16	4619	70	6023	89	0.494041	0
17	3389	76	4147	97	0.400369	0
18	1370	74	1884	94	0.576479	0
19	4277	74	2368	88	-0.771801	0

Total number of rows: 7085

Table truncated, full table size 211 Kbytes.

Supplementary data files not provided