RNA from a haploid F1 segregant from a cross between the lab strain BY4716 (isogenic to S288c) and haploid derivative RM11-1a from a wild isolate strain from a California vineyard collected by R. Mortimer. The reference sample is RNA from haploid BY4716. See Yvert et al. Nature Genetics 2003. NOTE: REFERENCE SAMPLE IS DIFFERENT FROM THE ONE USED IN Brem et al. Science 2002 296:752-755. It corresponds to the same strain but comes from an independent culture. Lot batch =
Trans-acting regulatory variation in Saccharomyces cerevisiae
Data table header descriptions
ID_REF
spot number from platform GPL118 (yeast cDNA arrays manufactured
CH1_MEAN
median signal at 635 nm
CH1_BKD_MEAN
median background signal at 635 nm
CH2_MEAN
median signal at 532 nm
CH2_BKD_MEAN
median background signal at 532 nm
VALUE
First compute l = log2 (median 635 - median background 635) - log2 (median 532 - median background 532). Do this for every spot on chip c. Next compute m, the mean of these l values over all spots on chip c. Now subtract m from every l value on chip c to get the final normalized log ratio n. If the SAMPLE has Cy3 in the name, n is the VALUE here; if the SAMPLE has Cy5 in the name, -n is the VALUE.
FLAG
GenePix flag; all spots with flag < 0 (defective spot or data) removed
