|
| Status |
Public on May 02, 2013 |
| Title |
T20 OSCC |
| Sample type |
RNA |
| |
|
| Source name |
oral squamous cell carcinoma
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: oral squamous cell carcinoma
|
| Extracted molecule |
total RNA |
| Extraction protocol |
OSCC and non-OSCC pair-wise samples were prepared after cutting into 5μm-thick sections on a microtome with a disposable blade.Total RNA was isolated using an RNeasy mini kit (Qiagen) according to the manufacturer's instruction.
|
| Label |
biotin
|
| Label protocol |
500ng total RNA was converted into cDNA using biotinylated random nonamers, oligodeoxythymidine 18 primers and Illumina-supplied reagents, according to Illumina manufacturer's instructions.
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
non-OSCC
|
| Data processing |
Data analysis was performed with GeneSpring GX software (Agilent Technologies). Arrays with less than 50% detected probes were excluded. Array data from 28 tumor and non-tumor pair-wise sample were then subjected to further analysis. Quantile normalisation was performed.
|
| |
|
| Submission date |
May 15, 2012 |
| Last update date |
May 18, 2015 |
| Contact name |
Chia Huei Lee |
| E-mail(s) |
chlee124@nhri.edu.tw
|
| Organization name |
Taiwan National Health Research Institute
|
| Department |
National Institute of Cancer Research
|
| Street address |
35, Keyan Rd.
|
| City |
Zhunan Miaoli County |
| ZIP/Postal code |
114 |
| Country |
Taiwan |
| |
|
| Platform ID |
GPL6883 |
| Series (1) |
| GSE37991 |
Gene expression profiling of oral squamous cell carcinoma (OSCC) |
|
