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Sample GSM9314

Query DataSets for GSM9314

Status

Public on Sep 03, 2003

Title

1-5-cCy5

Sample type

RNA

Channel 1

Source name

segregant from BY4716 x wild wine strain RM11-1a

Organism

Saccharomyces cerevisiae

Extracted molecule

total RNA

Channel 2

Source name

reference pool of BY4716 strain

Organism

Saccharomyces cerevisiae

Extracted molecule

total RNA

Description

RNA from a haploid F1 segregant from a cross between the
lab strain BY4716 (isogenic to S288c) and haploid derivative RM11-1a from a wild isolate
strain from a California vineyard collected by R.
Mortimer. The reference sample is RNA from haploid
BY4716. See Yvert et al. Nature Genetics 2003.
NOTE: REFERENCE SAMPLE IS DIFFERENT FROM THE ONE USED
IN Brem et al. Science 2002 296:752-755. It corresponds
to the same strain but comes from an independent culture.
Lot batch =

Submission date

Sep 02, 2003

Last update date

May 28, 2005

Contact name

Gael Yvert

E-mail(s)

yvert@insa-tlse.fr

Phone

011-33-5-61-55-94-25

Organization name

Fred Hutchinson Cancer Research Center

Lab

Kruglyak

Street address

City

Seattle

State/province

ZIP/Postal code

98109

Country

USA

Platform ID

GPL118

Series (1)

GSE617

Trans-acting regulatory variation in Saccharomyces cerevisiae

Data table header descriptions
ID_REF	spot number from platform GPL118 (yeast cDNA arrays manufactured
CH1_MEAN	median signal at 635 nm
CH1_BKD_MEAN	median background signal at 635 nm
CH2_MEAN	median signal at 532 nm
CH2_BKD_MEAN	median background signal at 532 nm
VALUE	First compute l = log2 (median 635 - median background 635) - log2 (median 532 - median background 532). Do this for every spot on chip c. Next compute m, the mean of these l values over all spots on chip c. Now subtract m from every l value on chip c to get the final normalized log ratio n. If the SAMPLE has Cy3 in the name, n is the VALUE here; if the SAMPLE has Cy5 in the name, -n is the VALUE.
FLAG	GenePix flag; all spots with flag < 0 (defective spot or data) removed

Data table
ID_REF	CH1_MEAN	CH1_BKD_MEAN	CH2_MEAN	CH2_BKD_MEAN	VALUE	FLAG
0	1647	91	2204	122	-0.242779	0
1	1225	93	1548	123	-0.154739	0
2	1010	93	1100	124	0.087390	0
3	989	92	1489	126	-0.426257	0
4	669	87	1030	119	-0.469083	0
5	1199	84	1575	109	-0.217492	0
6	243	77	313	103		-50
7	2059	77	2538	104	-0.119023	0
8	1996	78	2527	105	-0.159247	0
9	1230	81	1840	108	-0.414711	0
10	1796	95	2560	122	-0.341966	0
11	11150	90	12363	115	0.030154	0
12	8545	84	11377	111	-0.235726	0
13	4106	91	4992	118	-0.102357	0
14	1340	105	1819	134	-0.270889	0
15	5297	112	6877	143	-0.199771	0
16	6746	107	8607	138	-0.173877	0
17	4421	102	5873	132	-0.233256	0
18	2099	105	2320	134	0.044721	0
19	2957	117	8271	150	-1.338417	0

Total number of rows: 7085

Table truncated, full table size 215 Kbytes.

Supplementary data files not provided