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Sample GSM9304 Query DataSets for GSM9304
Status Public on Sep 03, 2003
Title RM1Cy5
Sample type RNA
 
Channel 1
Source name independent culture of wild strain RM11
Organism Saccharomyces cerevisiae
Extracted molecule total RNA
 
Channel 2
Source name reference pool of BY4716 strain
Organism Saccharomyces cerevisiae
Extracted molecule total RNA
 
 
Description RNA from the lab strain BY4716 (S288c general background).
and a haploid derivative of wild isolate strain (RM11) from a California vineyard
collected by R. Mortimer. See Yvert et al. Nature Genetics 2003.
NOTE: REFERENCE SAMPLE IS DIFFERENT FROM THE ONE USED
IN Brem et al. Science 2002 296:752-755. It corresponds
to the same strain but comes from an independent culture.
Lot batch =
 
Submission date Sep 02, 2003
Last update date May 28, 2005
Contact name Gael Yvert
E-mail(s) yvert@insa-tlse.fr
Phone 011-33-5-61-55-94-25
Organization name Fred Hutchinson Cancer Research Center
Lab Kruglyak
Street address
City Seattle
State/province WA
ZIP/Postal code 98109
Country USA
 
Platform ID GPL118
Series (1)
GSE617 Trans-acting regulatory variation in Saccharomyces cerevisiae

Data table header descriptions
ID_REF spot number from platform GPL118 (yeast cDNA arrays manufactured
CH1_MEAN median signal at 635 nm
CH1_BKD_MEAN median background signal at 635 nm
CH2_MEAN median signal at 532 nm
CH2_BKD_MEAN median background signal at 532 nm
VALUE First compute l = log2 (median 635 - median background 635) - log2 (median 532 - median background 532). Do this for every spot on chip c. Next compute m, the mean of these l values over all spots on chip c. Now subtract m from every l value on chip c to get the final normalized log ratio n. If the SAMPLE has Cy3 in the name, n is the VALUE here; if the SAMPLE has Cy5 in the name, -n is the VALUE.
FLAG GenePix flag; all spots with flag < 0 (defective spot or data) removed

Data table
ID_REF CH1_MEAN CH1_BKD_MEAN CH2_MEAN CH2_BKD_MEAN VALUE FLAG
0 837 35 964 43 -0.119414 0
1 369 35 598 44 -0.649853 0
2 222 35 453 45 -1.045346 0
3 646 36 688 46 0.006421 0
4 287 37 393 48 -0.384483 0
5 701 39 912 54 -0.293961 0
6 275 43 446 59 -0.658024 0
7 999 41 1083 53 -0.024362 0
8 699 41 1078 52 -0.560686 0
9 476 41 720 53 -0.536486 0
10 666 45 966 57 -0.469502 0
11 4045 59 4996 72 -0.224704 0
12 8039 74 4453 78 0.944576 0
13 1535 66 1774 70 -0.133906 0
14 538 52 642 60 -0.179878 0
15 2095 50 2636 59 -0.253407 0
16 2649 52 3254 62 -0.217430 0
17 1890 51 2181 62 -0.124277 0
18 704 53 923 66 -0.316453 0
19 1087 59 3461 79 -1.637851 0

Total number of rows: 7085

Table truncated, full table size 211 Kbytes.




Supplementary data files not provided
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