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Sample GSM927337 Query DataSets for GSM927337
Status Public on May 05, 2012
Title Moderate Restricted Carbohydrate Diet Supplemented Mouse 3
Sample type RNA
Source name Moderate Restricted Carbohydrate Diet Supplemented Mouse
Organism Mus musculus
Characteristics strain: C57BL/6
gender: male
tissue: liver tissue (non-tumor region)
disease model: human -HrasG12V / shp53/ GFP4 transposon vector induced transgenic mouse with liver cancer
Treatment protocol Following a preliminary feeding of each diet formula for one week, HrasG12V / shp53 / GFP4 gene containing transposon vector were injected into mouse tail vein by hydrodynamic injection method. After 5 weeks of diet supplementation, all mice were sacrificed. Mouse liver tissue was excised for microarray analysis.
Growth protocol Five- week- old male C57BL6 mice were randomly divided into two groups (n=3/group) and fed control diet (control group, LabDiet, Brentwood, MO, USA) or moderate restriced carbohydrate diet formula (MCD, Treat group) in a specific pathogen free zone. All procedures were approved by the institutional animal use and care committee.
Extracted molecule total RNA
Extraction protocol Non-tumor liver region was isolated from each mouse liver tissue. Total RNA extracted using Trizol following manufacturer's instructions.
Label Cy3
Label protocol Cyanine-3 (Cy3) labeled cRNA was prepared from 0.2 ug RNA using the One-Color Low RNA Input Linear Amplification PLUS kit (Agilent) according to the manufacturer's instructions, followed by RNAeasy column purification (QIAGEN, Valencia, CA). Dye incorporation and cRNA yield were checked with the NanoDrop ND-1000 Spectrophotometer.
Hybridization protocol 1.65 ug of Cy3-labelled cRNA (specific activity >6.0 pmol Cy3/ug cRNA) was fragmented at 60°C for 30 minutes in a reaction volume of 250 ul containing 1x Agilent fragmentation buffer and 2x Agilent blocking agent following the manufacturers instructions. On completion of the fragmentation reaction, 250 ul of 2x Agilent hybridization buffer was added to the fragmentation mixture and hybridized to Agilent Whole Mouse Genome Oligo Microarrays (G4122F) for 17 hours at 65°C in a rotating Agilent hybridization oven. After hybridization, microarrays were washed 1 minute at room temperature with GE Wash Buffer 1 (Agilent) and 1 minute with 37°C GE Wash buffer 2 (Agilent), then dried immediately by brief centrifugation.
Scan protocol Slides were scanned immediately after washing on the Agilent DNA Microarray Scanner (G2565AA) using one color scan setting.
Data processing Agilent Feature Extraction Software (v was used for background subtraction and LOWESS normalization.
Submission date May 04, 2012
Last update date May 05, 2012
Contact name Jong-Doo Lee
Phone 82-10-2829-1159
Fax 82-2-312-0578
Organization name Yonsei University
Street address 250 seonsan-ro seodaemum-gu
City Seoul
ZIP/Postal code 120-752
Country South Korea
Platform ID GPL11202
Series (1)
GSE37762 Analysis of differential genetic expression by moderate-carbohydrate restriction diet with calorie restriction mimetic multiple phytochemicals (MCDmp) using a transgenic liver cancer model developed by a simple hydrodynamic transfection method.

Data table header descriptions
VALUE Normalized signal intensity

Data table
A_51_P100034 1718.517
A_51_P100174 23.978338
A_51_P100208 2.531756
A_51_P100289 129.3999
A_51_P100298 3.4189467
A_51_P100309 2.156327
A_51_P100327 22.528599
A_51_P100347 9.623844
A_51_P100519 2.296934
A_51_P100537 6.2515903
A_51_P100573 43.66598
A_51_P100624 2.12204
A_51_P100625 4414.0693
A_51_P100768 2.066498
A_51_P100776 20.700888
A_51_P100787 364.10938
A_51_P100828 3241.0603
A_51_P100852 30.58089
A_51_P100991 54.73816
A_51_P100997 20.375181

Total number of rows: 39429

Table truncated, full table size 866 Kbytes.

Supplementary file Size Download File type/resource
GSM927337_GIII-3_006.txt.gz 9.0 Mb (ftp)(http) TXT
Processed data included within Sample table

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