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Sample GSM927334 Query DataSets for GSM927334
Status Public on May 05, 2012
Title Control Diet Supplemented Mouse 3
Sample type RNA
Source name Control Diet Supplemented
Organism Mus musculus
Characteristics strain: C57BL/6
gender: male
tissue: liver tissue (non-tumor region)
disease model: human -HrasG12V / shp53/ GFP4 transposon vector induced transgenic mouse with liver cancer
Treatment protocol Following a preliminary feeding of each diet formula for one week, HrasG12V / shp53 / GFP4 gene containing transposon vector were injected into mouse tail vein by hydrodynamic injection method. After 5 weeks of diet supplementation, all mice were sacrificed. Mouse liver tissue was excised for microarray analysis.
Growth protocol Five- week- old male C57BL6 mice were randomly divided into two groups (n=3/group) and fed control diet (control group, LabDiet, Brentwood, MO, USA) or moderate restriced carbohydrate diet formula (MCD, Treat group) in a specific pathogen free zone. All procedures were approved by the institutional animal use and care committee.
Extracted molecule total RNA
Extraction protocol Non-tumor liver region was isolated from each mouse liver tissue. Total RNA extracted using Trizol following manufacturer's instructions.
Label Cy3
Label protocol Cyanine-3 (Cy3) labeled cRNA was prepared from 0.2 ug RNA using the One-Color Low RNA Input Linear Amplification PLUS kit (Agilent) according to the manufacturer's instructions, followed by RNAeasy column purification (QIAGEN, Valencia, CA). Dye incorporation and cRNA yield were checked with the NanoDrop ND-1000 Spectrophotometer.
Hybridization protocol 1.65 ug of Cy3-labelled cRNA (specific activity >6.0 pmol Cy3/ug cRNA) was fragmented at 60°C for 30 minutes in a reaction volume of 250 ul containing 1x Agilent fragmentation buffer and 2x Agilent blocking agent following the manufacturers instructions. On completion of the fragmentation reaction, 250 ul of 2x Agilent hybridization buffer was added to the fragmentation mixture and hybridized to Agilent Whole Mouse Genome Oligo Microarrays (G4122F) for 17 hours at 65°C in a rotating Agilent hybridization oven. After hybridization, microarrays were washed 1 minute at room temperature with GE Wash Buffer 1 (Agilent) and 1 minute with 37°C GE Wash buffer 2 (Agilent), then dried immediately by brief centrifugation.
Scan protocol Slides were scanned immediately after washing on the Agilent DNA Microarray Scanner (G2565AA) using one color scan setting.
Data processing Agilent Feature Extraction Software (v was used for background subtraction and LOWESS normalization.
Submission date May 04, 2012
Last update date May 05, 2012
Contact name Jong-Doo Lee
Phone 82-10-2829-1159
Fax 82-2-312-0578
Organization name Yonsei University
Street address 250 seonsan-ro seodaemum-gu
City Seoul
ZIP/Postal code 120-752
Country South Korea
Platform ID GPL11202
Series (1)
GSE37762 Analysis of differential genetic expression by moderate-carbohydrate restriction diet with calorie restriction mimetic multiple phytochemicals (MCDmp) using a transgenic liver cancer model developed by a simple hydrodynamic transfection method.

Data table header descriptions
VALUE Normalized signal intensity

Data table
A_51_P100034 3190.497
A_51_P100174 48.730698
A_51_P100208 2.33521
A_51_P100289 152.3165
A_51_P100298 26.645477
A_51_P100309 2.0824506
A_51_P100327 35.65641
A_51_P100347 22.00744
A_51_P100519 1.992042
A_51_P100537 5.3290973
A_51_P100573 77.20243
A_51_P100624 1.84976
A_51_P100625 7771.2646
A_51_P100768 1.755821
A_51_P100776 49.58073
A_51_P100787 212.03271
A_51_P100828 2061.1155
A_51_P100852 42.645332
A_51_P100991 108.3789
A_51_P100997 22.766605

Total number of rows: 39429

Table truncated, full table size 866 Kbytes.

Supplementary file Size Download File type/resource
GSM927334_GI-3_003.txt.gz 9.0 Mb (ftp)(http) TXT
Processed data included within Sample table

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