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Sample GSM925225 Query DataSets for GSM925225
Status Public on May 02, 2012
Title baseline_volunteer3
Sample type RNA
 
Source name baseline_volunteer3
Organism Homo sapiens
Characteristics tissue: blood
Treatment protocol globin and DNAse treatment
Growth protocol RNA total from blood
Extracted molecule total RNA
Extraction protocol PAXgene kit
Label biotin
Label protocol IVT labeling kit
 
Hybridization protocol IVT kit (affymetrix)
Scan protocol GCOS
Description Human Genome Plus
Data processing Gene expression values were obtained using the three-step Robust Multi-array Average (RMA) pre-processing method (Irizarry et al., 2003), implemented in the Affymetrix Inc. package from R/Bioconductor. We applied the ComBat method prior to analysis for adjusting data for undesirable experimental variation (or batch effect) caused by different days in which the microarrays were processed. ComBat implements an empirical Bayes approach, that is commonly used in microarray analyses due to its ability to robustly handle high-dimensional data when sample sizes are small (Johnson et al, 2007).
 
Submission date Apr 30, 2012
Last update date May 02, 2012
Contact name Renata Pellegrino
E-mail(s) renata.pellegrino@gmail.com
Phone 2154327670
Organization name UNIFESP
Street address Rua NApoleao de Barros 925
City Sao Paulo
ZIP/Postal code 04024-002
Country Brazil
 
Platform ID GPL570
Series (1)
GSE37667 The peripheral genome-wide gene expression profiles in humans after prolonged wakefulness and sleep recovery

Data table header descriptions
ID_REF
VALUE RMA signal

Data table
ID_REF VALUE
1007_s_at 6.556372697
1053_at 6.960233579
117_at 9.155504441
121_at 6.73659204
1255_g_at 2.269568392
1294_at 8.386762883
1316_at 4.973227517
1320_at 2.649018193
1405_i_at 12.5379276
1431_at 2.764207951
1438_at 4.357561684
1487_at 7.756948648
1494_f_at 4.546582734
1552256_a_at 6.086662044
1552257_a_at 7.571715254
1552258_at 4.56058268
1552261_at 2.90309798
1552263_at 8.868208042
1552264_a_at 8.970783377
1552266_at 2.164362728

Total number of rows: 54675

Table truncated, full table size 1211 Kbytes.




Supplementary file Size Download File type/resource
GSM925225_Da_rebote.CEL.gz 4.6 Mb (ftp)(http) CEL
Processed data included within Sample table

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