|
Status |
Public on Apr 25, 2015 |
Title |
3100084 |
Sample type |
RNA |
|
|
Source name |
Human Peripheral Blood
|
Organism |
Homo sapiens |
Characteristics |
age: 91 tissue: Human Peripheral Blood cell type: PBMC patient number: Patient 30 gender: Female ethnicity: Caucasian disease state: Ischemic Stroke time: Baseline
|
Extracted molecule |
total RNA |
Extraction protocol |
Total mRNA was extracted from the Paxgene Blood RNA tubes using the Paxgene Blood RNA Kit, PreAnalytiX Qagen
|
Label |
Biotin
|
Label protocol |
Biotinylated, amplified RNA was generated from the Illumina TotalPrep amplification kit (Applied Biosystems)
|
|
|
Hybridization protocol |
Illumina Whole genome gene expression direct hybridization assay
|
Scan protocol |
Illumina Bead Station 500x, scanning conducted using standard protocol
|
Description |
SAMPLE 33_Patient 30 Baseline
|
Data processing |
Raw intensity values were saved in Illumina's Bead Studio program manager and then downloaded into GeneSpring Agilent GX v10, RMA normalization collated the probe data (background correction; quantile normalization; summarization) Matrix normalized is the RMA normalized data for each probe; non-normalized is the raw signal intensity for each probe.
|
|
|
Submission date |
Apr 25, 2012 |
Last update date |
Apr 25, 2015 |
Contact name |
Taura L. Barr |
E-mail(s) |
tlbarr@hsc.wvu.edu
|
Phone |
304-293-0503
|
Organization name |
West Virginia University
|
Department |
School of Nursing
|
Street address |
One Medical Center Drive, BMRB Rm 119
|
City |
Morgantown |
State/province |
WV |
ZIP/Postal code |
26506 |
Country |
USA |
|
|
Platform ID |
GPL6883 |
Series (1) |
GSE37587 |
Gene Expression Profiling of Ischemic Stroke at 0-24 hours and 24-48 hours after known onset of symptoms |
|