|
| Status |
Public on Apr 25, 2015 |
| Title |
3100031 |
| Sample type |
RNA |
| |
|
| Source name |
Human Peripheral Blood
|
| Organism |
Homo sapiens |
| Characteristics |
age: 84
tissue: Human Peripheral Blood
cell type: PBMC
patient number: Patient 1
gender: Male
ethnicity: Caucasian
disease state: Ischemic Stroke
time: Baseline
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total mRNA was extracted from the Paxgene Blood RNA tubes using the Paxgene Blood RNA Kit, PreAnalytiX Qagen
|
| Label |
Biotin
|
| Label protocol |
Biotinylated, amplified RNA was generated from the Illumina TotalPrep amplification kit (Applied Biosystems)
|
| |
|
| Hybridization protocol |
Illumina Whole genome gene expression direct hybridization assay
|
| Scan protocol |
Illumina Bead Station 500x, scanning conducted using standard protocol
|
| Description |
SAMPLE 12_Patient 1 Baseline
|
| Data processing |
Raw intensity values were saved in Illumina's Bead Studio program manager and then downloaded into GeneSpring Agilent GX v10, RMA normalization collated the probe data (background correction; quantile normalization; summarization)
Matrix normalized is the RMA normalized data for each probe; non-normalized is the raw signal intensity for each probe.
|
| |
|
| Submission date |
Apr 25, 2012 |
| Last update date |
Apr 25, 2015 |
| Contact name |
Taura L. Barr |
| E-mail(s) |
tlbarr@hsc.wvu.edu
|
| Phone |
304-293-0503
|
| Organization name |
West Virginia University
|
| Department |
School of Nursing
|
| Street address |
One Medical Center Drive, BMRB Rm 119
|
| City |
Morgantown |
| State/province |
WV |
| ZIP/Postal code |
26506 |
| Country |
USA |
| |
|
| Platform ID |
GPL6883 |
| Series (1) |
| GSE37587 |
Gene Expression Profiling of Ischemic Stroke at 0-24 hours and 24-48 hours after known onset of symptoms |
|
