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Sample GSM922319 Query DataSets for GSM922319
Status Public on Jan 01, 2014
Title CAG-TKO_1
Sample type RNA
Source name Cortical explant Rb-TKO cells labeled by pCAG-Cre
Organism Mus musculus
Characteristics strain: C57BL/6
cell type: cortical explant cell
genotype/variation: Rb-TKO
labeled by: pCAG-Cre
Extracted molecule total RNA
Extraction protocol 10 ng of the total RNA was purified by RNeasy Micro (QIAGEN)
Label Cy3
Label protocol Total RNA was amplified following One-Color Microarray-Based Gene Expression Analysis (Low Input Quick Amp Labeling), ver 6.0.
Hybridization protocol The amplified cRNA (1.5 µg) was hybridized on Agilent Whole mouse Genome Array (4 × 44K) ver 2.0 for 17 hours.
Scan protocol The Chips were scanned on a Agilent DNA Microarray Scanner using Feature Extraction software ver (Agilent).
Description Cortical explant Rb-TKO cells labeled by pCAG-Cre
Data processing Normalized by pergentile shift (75%) and baseline to median of all samples; GeneSpring software ver 11.5.
Entities were filtered based on their signal intensity value (40-100%), and then the standard deviation (< 0.1). Filtered data linked as supplementary file on Series record.
Submission date Apr 25, 2012
Last update date Jan 01, 2014
Contact name Itsuki Ajioka
Organization name Tokyo Medical and Dental University
Department Center for Brain Integration Research
Street address 1-5-45 Yushima, Bunkyo-ku
City Tokyo
ZIP/Postal code 113-8510
Country Japan
Platform ID GPL11202
Series (1)
GSE37577 Gene expression profile of cerebral cortical cells after Rb family inactivation in progenitors and neurons

Data table header descriptions
VALUE normalized signal intensity

Data table
GE_BrightCorner -0.018689632
DarkCorner 0.5287504
A_55_P1989846 0.5672846
A_55_P1991598 0.50364304
A_55_P2022211 -0.88693047
A_55_P1980764 0.18765211
A_55_P1964375 -0.6614957
A_51_P128876 -0.53488207
A_55_P2121042 0.50228834
A_52_P219230 0.5018244
A_51_P207591 0.47530937
A_55_P2131920 -0.46066737
A_55_P2404223 -0.5033252
A_55_P2101944 0.040310383
A_52_P358860 -0.45029664
A_51_P119031 -0.027671814
A_51_P309854 -0.21503067
A_51_P343900 0.34114552
A_51_P234359 -0.096292496
A_51_P487813 -0.12231016

Total number of rows: 39485

Table truncated, full table size 954 Kbytes.

Supplementary file Size Download File type/resource
GSM922319_04_cTKO1.txt.gz 2.2 Mb (ftp)(http) TXT
Processed data included within Sample table
Processed data are available on Series record

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