|
| Status |
Public on Apr 13, 2013 |
| Title |
JJN3_3_POM_24h |
| Sample type |
RNA |
| |
|
| Source name |
JJN3 Multiple Myeloma cell line
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: JJN3
treatment: Pomalidomide (CC-4047) (100 µM, 24h)
|
| Treatment protocol |
JJN3 cells were cultured in presence of 100 µM Pomalidomide (CC-4047) for 24 hours.
|
| Growth protocol |
JJN3 cell line was maintained in culture in RPMI 1640 medium supplemented with 10% heat-inactivated fetal bovine serum (FBS), Penicillin (100U/ml), Streptomycin (100µg/ml), L-Glutamine (2mM) and Fungizone antimycotic (2,5µg/ml) (Invitrogen Life Technologies, Milan, Italy).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from the cells using the RNeasy total RNA isolation kit (Qiagen, Valencia, CA), and then quantified using a Nanodrop ND-100 (Celbio S.p.A., Milan, Italy).
|
| Label |
biotin
|
| Label protocol |
Biotin labeling of antisense cRNA was performed according to standard Affymetrix protocols.
|
| |
|
| Hybridization protocol |
Biotin labeled cRNA target was hybridized on GeneChip® Human Genome U133 Plus 2.0 Arrays (Affymetrix Inc., Santa Clara, CA, USA) according to the standard Affymetrix protocol. The arrays were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
The GeneChip® Human Genome U133 Plus 2.0 arrays were scanned using the GeneChip® Scanner 3000 7G (Affymetrix Inc., Santa Clara, CA).
|
| Description |
Gene expression profiling data of Pomalidomide (CC-4047) treated JJN3 cell line replicate 3
|
| Data processing |
Expression values were extracted from cell intensity files, using GeneAnnot custom chip definition files. The CDF Packages for R/Bioconductor (http://www.bioconductor.org/) version 2.2.0 was based on GeneAnnot annotation Version 2.0 and publicly available at http://www.xlab.unimo.it/GA_CDF/. The data were normalized using Robust Multi-array Average procedure.
|
| |
|
| Submission date |
Apr 16, 2012 |
| Last update date |
Apr 13, 2013 |
| Contact name |
Luca Agnelli |
| E-mail(s) |
luca.agnelli@istitutotumori.mi.it, luca.agnelli@gmail.com
|
| Phone |
+390223903581
|
| Organization name |
IRCCS Istituto Nazionale dei Tumori
|
| Department |
Department of Advanced Diagnostics
|
| Street address |
Venezian 1
|
| City |
MILAN |
| ZIP/Postal code |
20133 |
| Country |
Italy |
| |
|
| Platform ID |
GPL15445 |
| Series (1) |
| GSE37302 |
Lenalidomide and Pomalidomide inhibit Multiple Myeloma-induced osteoclast formation and RANKL/OPG ratio in myeloma microenvironment targeting the expression of adhesion molecules. |
|
