|
| Status |
Public on Apr 13, 2012 |
| Title |
Caltech_ChipSeq_C2C12_TCF3_(SC-349)_Control_32bp_EqS_2.0pct_5d |
| Sample type |
SRA |
| |
|
| Source name |
C2C12
|
| Organism |
Mus musculus |
| Characteristics |
lab: Caltech-m
lab description: Wold - Califonia Institute of Technology
datatype: ChipSeq
datatype description: Chromatin IP Sequencing
cell: C2C12
cell organism: mouse
cell description: Myoblast cell line derived from thigh muscle of C3H mice after crush injury
cell sex: F
antibody: TCF3_(SC-349)
antibody description: E2A.E12(V-18) is an affinity purified rabbit polyclonal antibody raised against a peptide mapping at the C-terminus of E2A.E12 of human origin. Recommended for detection of E12 and E47 of m, r and h origin by WB, IP, IF, IHC(P), ELISA and indirect FCM using PE (sc-3739)- or FITC (sc-2012)-conjugated goat anti-rabbit IgG. Antibody Target: TCF3
antibody targetdescription: Heterodimers between TCF3 and tissue-specific basic helix-loop-helix (bHLH) proteins play major roles in determining tissue-specific cell fate during embryogenesis, like muscle or early B-cell differentiation. Dimers bind DNA on E-box motifs: 5'-CANNTG-3'. Binds to the kappa-E2 site in the kappa immunoglobulin gene enhancer.
antibody vendorname: Santa Cruz Biotech
antibody vendorid: sc-349
control: Control_32bp
control description: This data represents a control being compared to experiments using read length of 32 bp
treatment: EqS_2.0pct_5d
treatment description: 5 d with 2.0% Equine Serum and Insulin (Wold)
age: immortalized
age description: Immortal cells
control: Control_32bp
control description: This data represents a control being compared to experiments using read length of 32 bp
controlid: 10194
labexpid: 10409
labversion: Myocyte
labversion description: Myocyte
replicate: 1
strain: C3H
strain description: Strain C3H, Very related to C57BL/6
|
| Biomaterial provider |
Barbara Wold lab
|
| Treatment protocol |
EqS_2.0pct_5d
|
| Growth protocol |
C2C12_Wold_protocol.pdf
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
http://genome.ucsc.edu/cgi-bin/hgTrackUi?db=mm9&g=wgEncodeCaltechTfbs
|
| |
|
| Library strategy |
ChIP-Seq |
| Library source |
genomic |
| Library selection |
ChIP |
| Instrument model |
Illumina Genome Analyzer II |
| |
|
| Data processing |
http://genome.ucsc.edu/cgi-bin/hgTrackUi?db=mm9&g=wgEncodeCaltechTfbs
|
| |
|
| Submission date |
Apr 13, 2012 |
| Last update date |
May 15, 2019 |
| Contact name |
ENCODE DCC |
| E-mail(s) |
encode-help@lists.stanford.edu
|
| Organization name |
ENCODE DCC
|
| Street address |
300 Pasteur Dr
|
| City |
Stanford |
| State/province |
CA |
| ZIP/Postal code |
94305-5120 |
| Country |
USA |
| |
|
| Platform ID |
GPL9250 |
| Series (2) |
| GSE36024 |
Transcription Factor Binding Sites by ChIP-seq from ENCODE/Caltech |
| GSE49847 |
A comparative encyclopedia of DNA elements in the mouse genome |
|
| Relations |
| SRA |
SRX142528 |
| BioSample |
SAMN00857819 |
| Named Annotation |
GSM915177_mm9_wgEncodeCaltechTfbsC2c12Tcf3FCntrl32bE2p5dPcr2xSigRep1.bigWig |
