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Sample GSM896032 Query DataSets for GSM896032
Status Public on Dec 01, 2012
Title LERKO biological rep 3
Sample type RNA
Source name Liver selective ERalpha knockout mouse
Organism Mus musculus
Characteristics Sex: female
genotype: Liver selective ERalpha knockout mouse
tissue: liver
genetic background: C57BL/6J
Extracted molecule total RNA
Extraction protocol Total RNA was isolated from frozen mouse tissues using the Trizol reagent (Invitrogen) as per manufacturer’s instructions. The isolated RNA was subsequently purified with the RNeasy Plus Mini Kits (Qiagen),
Label biotin
Label protocol Fragmented single-stranded sense DNA was end labeled with biotin according to the Affymetrx Gene chip WT Terminal Labeling and hybridization user manual
Hybridization protocol The Gene Chip Mouse Gene 1.1 ST Array Plate was hybridized in the Affymetrix Gene Titan Instrument standard procedure
Scan protocol The Gene Chip Mouse Gene 1.1 ST Array Plate was scanned in Affymetrix Gene Titan Instrument standard procedure
Data processing The data were analyzed with oilgo package (version 1.16.0) in Bioconductor. Transcripts level were summaried for core probesets using RMA.
Submission date Mar 14, 2012
Last update date Dec 01, 2012
Contact name Marko Matic
Organization name Karolinska Institutet
Street address Halsovägen 7
City Huddinge
ZIP/Postal code 14183
Country Sweden
Platform ID GPL11533
Series (1)
GSE36514 Estrogen signaling and the Metabolic Syndrome: Targeting the hepatic ERalpha action

Data table header descriptions
VALUE log2 RMA signal

Data table
10338001 10.79533832
10338002 4.069196459
10338003 9.116746252
10338004 8.515953128
10338005 1.880362579
10338006 2.053742936
10338007 2.328829389
10338008 2.59030783
10338009 6.168405815
10338010 1.906884722
10338011 3.56328068
10338012 1.988960582
10338013 1.721208544
10338014 1.757286543
10338015 1.674971145
10338016 4.926907902
10338017 12.26099212
10338018 4.478249069
10338019 3.202054074
10338020 5.909248535

Total number of rows: 35556

Table truncated, full table size 725 Kbytes.

Supplementary file Size Download File type/resource
GSM896032_MM12_D07.CEL.gz 4.3 Mb (ftp)(http) CEL
Processed data included within Sample table

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