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Sample GSM879084 Query DataSets for GSM879084
Status Public on Nov 01, 2012
Title D2 11:20_SHAM exposure
Sample type RNA
 
Source name SHAM exposure
Organism Homo sapiens
Characteristics tissue source: Healthy volunteers
gender: male
age: 20-30 years
tissue: Peripheral blood
study day: Day2
time-point: 11:20
treatment: SHAM exposure (50 Hz EMF of 0.21 ± 0.05 μT for 2h)
Treatment protocol 17 healthy male subjects age 20 -30 each received an extremely low frequency electromagnetic field exposure (ELF-EMF) on study day 1 repeated on study day 3 (7 days later), a sham exposure ( using counterwound coils in the 1 M cube exposure frames) on study day 2 (the day after study day 1) and a null exposure (no current through coils) on study day 4 (the day after study day 3). All exposures (ELF-EMF, sham or null) were for 2 h at 11.00 - 13.00. 10 ml blood samples were collected at each time point by cannula at 9.00, 11.00, 11.05, 11.10, 11.20,11.40, 12.20, 13.00, 15.00 and 17.00) and transfered directly into PAXgene blood tubes.
Extracted molecule total RNA
Extraction protocol The whole blood samples collected in PAXgene blood tubes were stored a 4C overnight and the RNA was extracted the next day with the PAXgene Blood RNA kit from QIAGEN according to the manufacturer's instructions, including the DNase treatment. A pooling strategy was used to detect significant responses that occur in most or all of the volunteers. Equal amounts of RNA (300 ng/sample) were pooled from the 17 RNA samples samples for each of the 40 time points on study days 1 to 4. The 260/280 nm ratio of all pools was > 2.0. The 40 pooled RNA samples were assayed by Agilent Bioanalyser for RNA Integrity Number (RIN) and all RIN values were >8.0 (38/40>9.0).
Label biotin
Label protocol Biotinylated cRNA samples were prepared with the Illumina TotalPrep RNA Amplification kit from Ambion.
 
Hybridization protocol Standard Illumina hybridization protocol
Scan protocol Standard Illumina scanning protocol using the Beadarray reader.
Description SAMPLE 15
pooled RNA samples using equal amounts of RNA from the 17 volunteers
Data processing Beadstudio v.2.3.47 was used for processing the raw data and the Average method was used for normalization. Averaged data were filtered for probes with detection pvalue < 0.01 in at least one sample.
 
Submission date Feb 22, 2012
Last update date Nov 01, 2012
Contact name James Metcalfe
E-mail(s) jcm@mole.bio.cam.ac.uk
Organization name University of Cambridge
Department Biochemistry
Street address 80 Tennis Court Road
City Cambridge
ZIP/Postal code CB2 8PJ
Country United Kingdom
 
Platform ID GPL6097
Series (1)
GSE35999 Gene expression profiles in white blood cells of volunteers exposed to a 50 Hz electromagnetic field

Data table header descriptions
ID_REF
VALUE Average normalized signal intensity
Detection Pval

Data table
ID_REF VALUE Detection Pval
940746 108.7763 0
940731 1465.308 0
940707 393.9126 0
940692 134.8439 0
940673 18.59916 0.05009888
940671 712.4858 0
940632 210.4883 0
940600 24.90521 0.01779829
940592 95.47095 0
940577 83.52697 0
940576 25.48923 0.01450231
940546 60.30756 0
940504 514.4122 0
940500 304.2823 0
940494 94.17443 0
940487 582.0147 0
940463 2001.116 0
940452 126.1799 0
940451 90.99065 0
940435 71.43333 0

Total number of rows: 16736

Table truncated, full table size 390 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

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