|
| Status |
Public on Jun 18, 2012 |
| Title |
YFP-pos GMP Dicer het rep2 |
| Sample type |
RNA |
| |
|
| Source name |
YFP-pos GMPs
|
| Organism |
Mus musculus |
| Characteristics |
genetic background: Cebpa-Cre;R26-LSL-Eyfp;Dicer1wt/fl/Dicer1fl/fl (donror), C57BL/6 (recipient)
genotype: Dicer heterozygous deletion
cell type: YFP-positive granulocyte-macrophage progenitor
|
| Extracted molecule |
total RNA |
| Extraction protocol |
EYFP positive GMPs were sorted into RLT buffer (Qiagen, Venlo, the Netherlands). RNA was extracted with the RNeasy Micro Kit (Qiagen).
|
| Label |
Biotin
|
| Label protocol |
standard Affymetrix protocol, eukaryotic Target Labeling Assay
|
| |
|
| Hybridization protocol |
standard Affymetrix protocol
|
| Scan protocol |
standard Affymetrix protocol, Affymetrix 7G
|
| Description |
C57BL/6, Female, bonemarrow, GMPs
Dicer heterozygous deletion
|
| Data processing |
Microarray data were normalized with Affymetrix Microarray Suite (MAS 5.0)
|
| |
|
| Submission date |
Feb 15, 2012 |
| Last update date |
Jun 18, 2012 |
| Contact name |
Stefan J Erkeland |
| E-mail(s) |
s.erkeland@erasmusmc.nl
|
| Phone |
+31(0)107043034
|
| Fax |
+31(0)107044745
|
| Organization name |
ErasmusMC
|
| Department |
Hematology
|
| Lab |
1330
|
| Street address |
Dr. Molewaterplein 50
|
| City |
Rotterdam |
| ZIP/Postal code |
3015GE |
| Country |
Netherlands |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE35844 |
Dicer1 deletion in myeloid-committed progenitors causes neutrophil dysplasia and blocks macrophage/dendritic cell development in mice |
|
