|
| Status |
Public on Jul 05, 2012 |
| Title |
gastric tumor Gastric0091 |
| Sample type |
RNA |
| |
|
| Source name |
gastric tumor Gastric0091
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: gastric adenocarcinoma
subtype: Proliferative
|
| Treatment protocol |
gastric tumor tissue.
|
| Growth protocol |
gastric tumor tissue.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from primary tumors using RNeasy mini spin columns (Qiagen) according to the instructions of the manufacturer.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 to 5 ug total RNA (Expression Analysis Technical Manual, 2006, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip® Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner GCS3000 7G.
|
| Description |
gene expression data from gastric tumor Gastric0091
|
| Data processing |
The data were RMA normalized with R package affy.
|
| |
|
| Submission date |
Feb 14, 2012 |
| Last update date |
Jul 05, 2012 |
| Contact name |
Zhengdeng Lei |
| E-mail(s) |
zhengdeng.lei@duke-nus.edu.sg
|
| Organization name |
Duke-NUS graduate medical school
|
| Lab |
Lei
|
| Street address |
8 College Road
|
| City |
Singapore |
| ZIP/Postal code |
169857 |
| Country |
Singapore |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE35809 |
Gastric Cancer Subtyping (Australian Patient Cohort) |
|
