Genomic DNA was extracted from whole bone marrow, whole peripheral blood or CD3 lymphocytes isolated from peripheral blood prior to treatment with lenalidomide, at the individual centers participating in the study. DNA quality and quantity was assessed using a Nanodrop Spectrophotometer and agarose gel electrophoresis.
Label
biotin
Label protocol
DNA was restriction digested, PCR amplified, fragmented, labeled and hybridized to each array according to the manufacturer's instructions.
Hybridization protocol
The Arrays were then washed using Affymetrix fluidics stations, and scanned according to the manufacturer's instructions.
Scan protocol
CEL files, generated by AGCC Viewer. CNCHP and LOHCHP, generated by Genotyping Console (all provided by Affymetrix)
Description
Hybridized to 6.0 Array
Data processing
CNCHP (from 6.0 arrays) provide data for copy number and LOH analysis. Genotyping Console; algorithm-name = CN5
Submission date
Feb 09, 2012
Last update date
Jul 01, 2013
Contact name
Mar Mallo
Organization name
Josep Carreras Leukaemia Research Institute
Street address
Ctra de Can Ruti, s/n, camà de les escoles. Edifici IMPPC
