|
| Status |
Public on Mar 19, 2015 |
| Title |
WTBapx1 Vertebral Column Cells-Rep2 |
| Sample type |
RNA |
| |
|
| Source name |
WTBapx1 Vertebral Column Cells-Rep2
|
| Organism |
Mus musculus |
| Characteristics |
developmental stage: E12.5
tissue: Verebral Column
strain: C57BL/6J;129
genotype/variation: Bapx1 WT
|
| Treatment protocol |
Vertebral column was dissected out of WT embryos where was Bapx1 gene was tagged with EGFP at the 3' UTR vis IRES and homozygous embryos where Bapx1 was knocked out by EGFP nadsubjected to Fluoroscent activated cell sorting
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from the sorted cells by Trizol followed by DNAse treatment and QIAGEN RNeasy kit as per manufacturer's instruction
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA samples were prepared using the TargetAmp-Nano labelling kit for Illumina Arrays
|
| |
|
| Hybridization protocol |
Standard Illumina Hyb
|
| Scan protocol |
Standard Illumina Scan
|
| Data processing |
Illumina output was imported to Genome Studio and no normalization was done. Genespring(Gx 11.0) was used to do normalize the data using the following parameters- Normalization Algorithm-Scale. Scale to median of all samples. Threshold raw signal to 1.
|
| |
|
| Submission date |
Feb 09, 2012 |
| Last update date |
Mar 19, 2015 |
| Contact name |
Thomas Lufkin |
| E-mail(s) |
tlufkin@clarkson.edu
|
| Organization name |
Clarkson University
|
| Department |
Biology
|
| Lab |
Stem Cells & Regenerative Medicine
|
| Street address |
8 Clarkson Avenue
|
| City |
Potsdam |
| State/province |
NY |
| ZIP/Postal code |
13699 |
| Country |
USA |
| |
|
| Platform ID |
GPL6887 |
| Series (2) |
| GSE35655 |
Gene Expression profiles of Bapx1 sorted cells from vertebral column of wildtype (WT) and Bapx1 null (HOMO) E12.5 mouse embryos |
| GSE35877 |
Genome wide binding and expression profiling defines a Bapx1-Sox9 axis in vertebral column, Gut, Spleen and limbs in developing mouse embryo. |
|
