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Status |
Public on Jul 01, 2012 |
Title |
NEUROB-3-2 |
Sample type |
RNA |
|
|
Source name |
MSCs from NB patient 3
|
Organism |
Homo sapiens |
Characteristics |
diagnosis: neuroblastoma cell type: mesenchymal stem cells replicate: replicate 2
|
Growth protocol |
Bone marrow-derived MSCs were obtained by adherence to plastic. Mononucleated cells were obtained after centrifugation using a Ficoll-Paque gradient. Cells were cultured at 37°C with 5% CO2 in DMEM (Lonza) supplemented with 10% Fetal Bovine Serum
|
Extracted molecule |
total RNA |
Extraction protocol |
MSCs were stabilized in PrepProtect™ (Miltenyi Biotec) and total RNA was isolated using standard RNA extraction protocols (NucleoSpin® RNA II, Macherey-Nagel).
|
Label |
Cy3
|
Label protocol |
To produce Cy3-labeled cRNA, the RNA samples were amplified and labelled using the Agilent Low RNA Input Linear Amp Kit (Agilent Technologies) following the manufacturer’s protocol.
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Hybridization protocol |
1.65 μg Cy3-labeled fragmented cRNA in hybridization buffer was hybridized overnight (17 hours, 65°C) to Agilent Whole Human Genome Oligo Microarrays 4×44K using Agilent’s recommended hybridization chamber and oven.
|
Scan protocol |
Fluorescence signals of the hybridized Agilent Microarrays were detected using Agilent’s Microarray Scanner System (Agilent Technologies).
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Description |
Gene expression of MSCs from NB patient
|
Data processing |
The Agilent Feature Extraction Software (FES) was used to read out and process the microarray image files.
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|
|
Submission date |
Jan 17, 2012 |
Last update date |
Jul 01, 2012 |
Contact name |
Javier Garcia-Castro |
E-mail(s) |
jgcastro@isciii.es
|
Phone |
34-91-8223261
|
Organization name |
Instituto de Salud Carlos III
|
Department |
Centro Nacional de Microbiología
|
Lab |
Unidad de Biotecnología Celular
|
Street address |
Ctra. Majadahonda-Pozuelo km 2,2
|
City |
Majadahonda |
State/province |
Madrid |
ZIP/Postal code |
28220 |
Country |
Spain |
|
|
Platform ID |
GPL4133 |
Series (1) |
GSE35133 |
Development of gene expression signatures for mesenchymal stem cells from neuroblastoma patients |
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