|
| Status |
Public on Jul 01, 2012 |
| Title |
CONTROL-3-3 |
| Sample type |
RNA |
| |
|
| Source name |
MSCs from healthy donor 3
|
| Organism |
Homo sapiens |
| Characteristics |
diagnosis: healthy donor
cell type: mesenchymal stem cells
replicate: replicate 3
|
| Growth protocol |
Bone marrow-derived MSCs were obtained by adherence to plastic. Mononucleated cells were obtained after centrifugation using a Ficoll-Paque gradient. Cells were cultured at 37°C with 5% CO2 in DMEM (Lonza) supplemented with 10% Fetal Bovine Serum
|
| Extracted molecule |
total RNA |
| Extraction protocol |
MSCs were stabilized in PrepProtect™ (Miltenyi Biotec) and total RNA was isolated using standard RNA extraction protocols (NucleoSpin® RNA II, Macherey-Nagel).
|
| Label |
Cy3
|
| Label protocol |
To produce Cy3-labeled cRNA, the RNA samples were amplified and labelled using the Agilent Low RNA Input Linear Amp Kit (Agilent Technologies) following the manufacturer’s protocol.
|
| |
|
| Hybridization protocol |
1.65 μg Cy3-labeled fragmented cRNA in hybridization buffer was hybridized overnight (17 hours, 65°C) to Agilent Whole Human Genome Oligo Microarrays 4×44K using Agilent’s recommended hybridization chamber and oven.
|
| Scan protocol |
Fluorescence signals of the hybridized Agilent Microarrays were detected using Agilent’s Microarray Scanner System (Agilent Technologies).
|
| Description |
Gene expression of MSCs from healthy donor
|
| Data processing |
The Agilent Feature Extraction Software (FES) was used to read out and process the microarray image files.
|
| |
|
| Submission date |
Jan 17, 2012 |
| Last update date |
Jul 01, 2012 |
| Contact name |
Javier Garcia-Castro |
| E-mail(s) |
jgcastro@isciii.es
|
| Phone |
34-91-8223261
|
| Organization name |
Instituto de Salud Carlos III
|
| Department |
Centro Nacional de Microbiología
|
| Lab |
Unidad de Biotecnología Celular
|
| Street address |
Ctra. Majadahonda-Pozuelo km 2,2
|
| City |
Majadahonda |
| State/province |
Madrid |
| ZIP/Postal code |
28220 |
| Country |
Spain |
| |
|
| Platform ID |
GPL4133 |
| Series (1) |
| GSE35133 |
Development of gene expression signatures for mesenchymal stem cells from neuroblastoma patients |
|
