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Sample GSM85812 Query DataSets for GSM85812
Status Public on Feb 28, 2006
Title Vd2 LPS rep1
Sample type RNA
Source name expanded peripheral blood Vd2 gd T lymphocytes
Organism Homo sapiens
Characteristics healthy male, age 27
Biomaterial provider donor 1
Treatment protocol phenol extracted E. coli lipopolysaccharide, 10 mg/mL, 4hr
Growth protocol 3 week culture with Vd1 mab 0.1mg/mL, pan gd mAb 0.1 mg/mL, and IL-15 10ng/mL
Extracted molecule total RNA
Extraction protocol cells were homogenized in TRIzol reagent, isopropanol precipitated, DNAse treated, then ethanol precipitated
Label biotin
Label protocol 1mg of total RNA was labelled using the Affymetrix One-Cycle labelling procedure
Hybridization protocol 15mg of labelled RNA was hybridized to the arrays according to standart Affymetrix procedures, then arrays were washed and stained on the Affymetrix GeneChip Fluidics Station 450 using the Midi_euk2v3 protocol
Scan protocol samples were scanned using the Affymetrix GeneChip Scanner 3000
Description expanded Vd2 cells, LPS treated, donor 1
Data processing Affymetrix GCOS
Submission date Dec 02, 2005
Last update date Dec 02, 2005
Contact name Ellen Kress
Phone 406-994-6384
Organization name Montana State University
Department Veterinary Molecular Biology
Lab Jutila
Street address PO Box 173610
City Bozeman
State/province MT
ZIP/Postal code 59717-3610
Country USA
Platform ID GPL571
Series (1)
GSE3720 Distinct gene expression in human Vd1 and Vd2 gd T cells following non-TCR agonist stimulation

Data table header descriptions
ID_REF Affymetrix probe set
VALUE signal intensity
ABS_CALL P for present, M for marginal, A for absent
DETECTION P-VALUE indicates the significance level of the P,A, or M absolute call

Data table
AFFX-BioB-5_at 538 P 0.000127
AFFX-BioB-M_at 813.4 P 0.000044
AFFX-BioB-3_at 465.5 P 0.000044
AFFX-BioC-5_at 1433 P 0.000052
AFFX-BioC-3_at 1874.7 P 0.000044
AFFX-BioDn-5_at 3379.8 P 0.000044
AFFX-BioDn-3_at 5844.2 P 0.000044
AFFX-CreX-5_at 15215.6 P 0.000052
AFFX-CreX-3_at 18857.7 P 0.000044
AFFX-DapX-5_at 193.4 P 0.000095
AFFX-DapX-M_at 374.7 P 0.001102
AFFX-DapX-3_at 421.9 P 0.00007
AFFX-LysX-5_at 19.4 P 0.000147
AFFX-LysX-M_at 43.5 P 0.036569
AFFX-LysX-3_at 92.7 P 0.00006
AFFX-PheX-5_at 40.7 P 0.000972
AFFX-PheX-M_at 64.7 P 0.002023
AFFX-PheX-3_at 89.2 P 0.000258
AFFX-ThrX-5_at 53.7 P 0.000509
AFFX-ThrX-M_at 64.8 P 0.000581

Total number of rows: 22277

Table truncated, full table size 588 Kbytes.

Supplementary data files not provided

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