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Sample GSM85804 Query DataSets for GSM85804
Status Public on Feb 28, 2006
Title Vd1 PBS rep1
Sample type RNA
Source name expanded peripheral blood Vd1 gd T lymphocytes
Organism Homo sapiens
Characteristics healthy male, age 27
Biomaterial provider donor 1
Treatment protocol PBS, 4hr
Growth protocol 3 week culture with Vd1 mab 0.1mg/mL, pan gd mAb 0.1 mg/mL, and IL-15 10ng/mL
Extracted molecule total RNA
Extraction protocol cells were homogenized in TRIzol reagent, isopropanol precipitated, DNAse treated, then ethanol precipitated
Label biotin
Label protocol 1mg of total RNA was labelled using the Affymetrix One-Cycle labelling procedure
Hybridization protocol 15mg of labelled RNA was hybridized to the arrays according to standart Affymetrix procedures, then arrays were washed and stained on the Affymetrix GeneChip Fluidics Station 450 using the Midi_euk2v3 protocol
Scan protocol samples were scanned using the Affymetrix GeneChip Scanner 3000
Description expanded Vd1 cells, PBS treated, donor 1
Data processing Affymetrix GCOS
Submission date Dec 02, 2005
Last update date Dec 02, 2005
Contact name Ellen Kress
Phone 406-994-6384
Organization name Montana State University
Department Veterinary Molecular Biology
Lab Jutila
Street address PO Box 173610
City Bozeman
State/province MT
ZIP/Postal code 59717-3610
Country USA
Platform ID GPL571
Series (1)
GSE3720 Distinct gene expression in human Vd1 and Vd2 gd T cells following non-TCR agonist stimulation

Data table header descriptions
ID_REF Affymetrix probe set
VALUE signal intensity
ABS_CALL P for present, M for marginal, A for absent
DETECTION P-VALUE indicates the significance level of the P,A, or M absolute call

Data table
AFFX-BioB-5_at 539.8 P 0.000095
AFFX-BioB-M_at 805.2 P 0.000044
AFFX-BioB-3_at 448.3 P 0.000044
AFFX-BioC-5_at 1406.4 P 0.000052
AFFX-BioC-3_at 1670.9 P 0.000044
AFFX-BioDn-5_at 3205.7 P 0.000044
AFFX-BioDn-3_at 5729.9 P 0.000044
AFFX-CreX-5_at 14456.1 P 0.000044
AFFX-CreX-3_at 17221 P 0.000044
AFFX-DapX-5_at 148.8 P 0.000081
AFFX-DapX-M_at 391.9 P 0.000754
AFFX-DapX-3_at 622.5 P 0.000052
AFFX-LysX-5_at 18.4 P 0.028457
AFFX-LysX-M_at 41.8 A 0.102165
AFFX-LysX-3_at 109.3 P 0.000095
AFFX-PheX-5_at 44.9 P 0.000147
AFFX-PheX-M_at 49.6 P 0.000972
AFFX-PheX-3_at 105.2 P 0.00034
AFFX-ThrX-5_at 44.3 P 0.009337
AFFX-ThrX-M_at 76 P 0.00039

Total number of rows: 22277

Table truncated, full table size 588 Kbytes.

Supplementary data files not provided

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