|
| Status |
Public on Jan 07, 2012 |
| Title |
BM_CDP_rep2 |
| Sample type |
RNA |
| |
|
| Source name |
mouse bone marrow
|
| Organism |
Mus musculus |
| Characteristics |
tissue: bone marrow
strain: C57BL/6
genotype: wild type
cell type: common dendritic cell progenitors (Lin-c-kitintSca-1-CD34+Flk2+CD16/32-CD115+)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
CDP or CMP were double sorted from the bone marrow of C57BL/6 mice. Total RNA was extracted using Trizol (Invitrogen) and linear acrylamide (Ambion) according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
RNA was amplified and biotin-lableled using the amplified using Affymetrix Two-Cycle Amplification and IVT kits (Affymetrix).
|
| |
|
| Hybridization protocol |
Used standard Affymetrix hybiridization protocol
|
| Scan protocol |
Used standard Affymetrix scanning protocol.
|
| Description |
replicate 2
|
| Data processing |
Background subtraction and quantiles-based normalization was performed using the GCRMA algorithm, available from Bioconductor.org.
|
| |
|
| Submission date |
Jan 05, 2012 |
| Last update date |
Jan 07, 2012 |
| Contact name |
Amy M. Becker |
| E-mail(s) |
abecker@path.wustl.edu
|
| Phone |
314-362-8782
|
| Fax |
314-362-8888
|
| Organization name |
Washington University School of Medicine
|
| Department |
Pathology and Immunology
|
| Lab |
Deepta Bhattacharya
|
| Street address |
660 S. Euclide Ave.
|
| City |
St. Louis |
| State/province |
MO |
| ZIP/Postal code |
63110 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (2) |
| GSE34892 |
IRF-8 extinguishes neutrophil production and promotes dendritic cell lineage commitment in both myeloid and lymphoid progenitors (Affymetrix). |
| GSE34917 |
IRF-8 extinguishes neutrophil production and promotes dendritic cell lineage commitment in both myeloid and lymphoid progenitors |
|
