Assays to confirm viability: in vivo transplantation assys
Extracted molecule
total RNA
Extraction protocol
RNA prep method: Arcturus pico pure Amplification method: N/A Cell purification method: Negative selection using magnets followed by FACS Contaminating cell type: N/A Method for estimating purity: FACS and in vitro colony assay
Label
Biotin
Description
This sample was analyzed as part of the Stem Cell Genomics Project (http://www.scgp.ca:8080/StemBase/). The biological material was submitted to the Ontario Genomics Innovation Centre (http://www.ottawagenomecenter.ca/) by Dr. Connie Eaves (ceaves@bccrc.ca; ) for analysis. Stembase Experiment ID: E232 Stembase Experiment ID link: http://www.scgp.ca:8080/StemBase/?path=/browse/experiment&id=232 SCGP Sample ID: S313 SCGP Sample ID link: http://www.scgp.ca:8080/StemBase/?path=/browse/experiment&id=232#SAMPLE_341 Short description: The sample is RNA derived from highly purified mouse mammary stem cells (Masc). These cells were derived from freshly dissociated mouse mammary tissue and were analyzed for cell content by in vivo transplantation arrays. The relevance to the stem cell project is that we have also isolated a mammary cell highly enriched in progenitor (CFC's). A comparison of the two population would be helpful in identifying stem cell specific genes. Estimated purity: 1 stem cell in every 50-100 cells RNA concentration: 534 Num cells for RNA prep: 2000-4000 Sample volume: ~20 ul
Data processing
Calculated using the MAS5 algorithm where sc=1500, tau=0.015, alpha1=0.04, and alpla2=0.06
