Assays to confirm viability: colony assays,in vivo transplantation assays
Extracted molecule
total RNA
Extraction protocol
RNA prep method: Arcturus pico pure Amplification method: N/A Cell purification method: Immunomagnetic enrichment and FACS Contaminating cell type: N/A Method for estimating purity: FACS and in vitro colony assay
Label
Biotin
Description
This sample was analyzed as part of the Stem Cell Genomics Project (http://www.scgp.ca:8080/StemBase/). The biological material was submitted to the Ontario Genomics Innovation Centre (http://www.ottawagenomecenter.ca/) by Dr. Connie Eaves (ceaves@bccrc.ca; ) for analysis. Stembase Experiment ID: E232 Stembase Experiment ID link: http://www.scgp.ca:8080/StemBase/?path=/browse/experiment&id=232 SCGP Sample ID: S312 SCGP Sample ID link: http://www.scgp.ca:8080/StemBase/?path=/browse/experiment&id=232#SAMPLE_340 Short description: The sample is RNA derived from mouse mammary myoepithelial (basal-positioned)cells. These cells are phenotipically similar to mouse mammary stem cells, but are deficient in stem ell contect. Consequentially, these are very useful in identifying stem cell specific genes. Estimated purity: ~94% RNA concentration: 823 Num cells for RNA prep: 3000- 24000 Sample volume: ~20 ul
Data processing
Calculated using the MAS5 algorithm where sc=1500, tau=0.015, alpha1=0.04, and alpla2=0.06
