|
| Status |
Public on Nov 06, 2012 |
| Title |
PCNSL patient 27 |
| Sample type |
RNA |
| |
|
| Source name |
Human brain tumor tissue from PCNSL patient
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: brain tumor
disease state: primary central nervous system lymphoma (PCNSL)
gender: male
age: 44 years
karnofsky performance status (kps): 50
therapy: HD-MTX chemotherapy
outcome: dead
survival time: 2-3 years
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 1 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome U133 Plus 2.0 Expression arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
Tumor tissue from male patient, 44 years old, KPS 50, HD-MTX chemotherapy treated.
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as the normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100. The data were log2-transformed.
|
| |
|
| Submission date |
Dec 29, 2011 |
| Last update date |
Nov 06, 2012 |
| Contact name |
Ryuya Yamanaka |
| E-mail(s) |
ryaman@koto.kpu-m.ac.jp
|
| Organization name |
Kyoto Prefectural University of Medicine
|
| Lab |
Molecular target therapy for cancer
|
| Street address |
465 Kajii-cho, Kamigyoku
|
| City |
Kyoto |
| ZIP/Postal code |
602-8566 |
| Country |
Japan |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE34771 |
Expression data from primary central nervous system lymphoma (PCNSL) patients |
|
