|
| Status |
Public on Oct 09, 2024 |
| Title |
Male_LPS rep1 |
| Sample type |
SRA |
| |
|
| Source name |
Whole brain
|
| Organism |
Mus musculus |
| Characteristics |
tissue: Whole brain
genotype: WT
treatment: Maternal LPS injection
|
| Treatment protocol |
The female mice (16th day of pregnancy) received an intraperitoneal injection with LPS (150ug/kg) or saline (150ug/kg). For each treatment group, 5 whole brain RNA samples of the male offspring (postnatal 56 days) were prepared.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using the RNeasy Plus Universal Mini Kit (Qiagen) according to the instruction.
RNA libraries for RNA-seq were prepared using NEBNext UltraTM RNA Library Prep Kit following manufacturer's protocols.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Description |
all_samples_rpkm.txt
|
| Data processing |
Quality control was performed by FastQC (v0.11.8) with passing all criteria.
Sequence reads were aligned to mouse genome MM10 (UCSC) using STAR (v2.7.1a) under the default parameters.
Aligned reads were quantified using HTSeq-counts (v0.11.2)
Low and un-expressed genes with < 3 reads in at least 5 samples in a group were omitted from the analysis
Assembly: mm10
Supplementary files format and content: tab-delimited text files include RPKM values for all Samples
|
| |
|
| Submission date |
Aug 15, 2024 |
| Last update date |
Oct 09, 2024 |
| Contact name |
Wenjun Yu |
| E-mail(s) |
wjyu0711@smu.edu.cn
|
| Organization name |
Center for Genetics and Developmental Systems Biology, Department of Obstetrics and Gynecology
|
| Street address |
Nanfang Hospital, Southern Medical University
|
| City |
Guangzhou |
| ZIP/Postal code |
510515 |
| Country |
China |
| |
|
| Platform ID |
GPL24247 |
| Series (1) |
| GSE233424 |
Maternal immune activation upregulates the AU020206-IRFs-STAT1 axis in modulating cytokine production in the brain |
|
| Relations |
| BioSample |
SAMN43203420 |
| SRA |
SRX25714014 |
