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Sample GSM8406610

Query DataSets for GSM8406610

Status

Public on Aug 19, 2024

Title

KGN-H3K27Ac-T-INPUT

Sample type

SRA

Source name

ovarian

Organism

Homo sapiens

Characteristics

tissue: ovarian
cell line: KGN
cell type: immortalized human granulosa cell
genotype: H3K27AC
treatment: 10 um testosterone for 24 h

Treatment protocol

10 um testosterone for 24 h

Growth protocol

DMEM-F12 with 10% FBS

Extracted molecule

genomic DNA

Extraction protocol

Cross-linked with a final concentration of 1.5% formaldehyde
TruSeq DNA LT/HT Sample Prep Kit (Illumina) and underwent quality control on the Bioanalyzer (Agilent 2200).

Library strategy

ChIP-Seq

Library source

genomic

Library selection

ChIP

Instrument model

Illumina HiSeq 2000

Data processing

The ChIP-seq reads were aligned to the human genome mm10 using Bowtie (Version 2.0) with default parameters.
For peak calling, MACS2.0 was employed with default parameters and a significance threshold (q value cutoff = 0.05), as described previously,
To visualize the ChIP-seq data, deepTools2 was utilized, BAM coverage was used to generate the bigwig files.
The genome tracks were visualized in the software program IGV. in different samples.
Peak annotation was performed using R and the ChIPseeker package and bedtools were employed to analyze the overlapping regions
Assembly: hg38
Supplementary files format and content: excel, narrow peak

Submission date

Jul 18, 2024

Last update date

Aug 19, 2024

Contact name

JIAMIN JIN

E-mail(s)

11718252@zju.edu.cn

Phone

18768117135

Organization name

ZheJiang University