 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Jun 18, 2012 |
| Title |
Oocyte litter 2 |
| Sample type |
SRA |
| |
|
| Source name |
oocyte
|
| Organism |
Mus musculus |
| Characteristics |
genetic background: mixed strain oocytes
cell type: oocyte
crosses: LSD1 FL/FL mice (From Wang et al, Nature 446, 2007 ) were crossed with ZP3::Cre mice (Jax labs strain 003651) and backcrossed again to generate LSD1 FL/FL, ZP3Cre+ and - mice. FOr this study, only LSD1 FL/FL, ZP3::Cre negative females were used to produce oocytes. For 2C embryos, these females were crossed with wild type CB6 males
|
| Treatment protocol |
Oocytes were collected by E0.5 and lysed in Prelude Direct Lysis Buffer
|
| Growth protocol |
Females were superovulated using standard conditions
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Nugen Ovation RNA-Seq kit(for amplifying RNA) followed by library construction using Illumina mRNA sample prep kit starting with end repair
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina Genome Analyzer II |
| |
|
| Description |
RNA amplified using SPIA
|
| Data processing |
Oocyte_2.wig; genome build: mm9
Bowtie alignments, allowing 3 mismatches per read, allowing up to 20 alignments per read
|
| |
|
| Submission date |
Nov 23, 2011 |
| Last update date |
May 15, 2019 |
| Contact name |
Todd Macfarlan |
| E-mail(s) |
macfarlan@salk.edu
|
| Organization name |
The Salk Institute
|
| Street address |
10010 North Torrey Pines
|
| City |
La Jolla |
| State/province |
CA |
| ZIP/Postal code |
92037 |
| Country |
USA |
| |
|
| Platform ID |
GPL9250 |
| Series (2) |
| GSE33922 |
RNA-Seq from wt oocytes |
| GSE33923 |
2C::tomato ES cells, 2-cell embryos and wild type oocytes |
|
| Relations |
| SRA |
SRX109435 |
| BioSample |
SAMN00760874 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSM838744_Oocyte_2.bed.gz |
48.1 Mb |
(ftp)(http) |
BED |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
|
|
|
|
 |
