|
| Status |
Public on Nov 23, 2012 |
| Title |
HCT116p53+/+ cells untreated 1 |
| Sample type |
RNA |
| |
|
| Source name |
HCT116p53+/+ cells untreated
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: HCT116p53+/+
cell type: colon cancer
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from frozen tissue samples using TRIZOL reagent (Invitrogen).
|
| Label |
biotin
|
| Label protocol |
Initially, we converted 250-500 ng total RNA to cDNA, followed by in vitro transcription to generate biotin-labeled cRNA (Illumina TotalPrep RNA Amplification Kit, Ambion Inc., Austin, TX).
|
| |
|
| Hybridization protocol |
The labeled probes were then mixed with hybridization reagents and hybridized overnight to the HumanHT-12 v3 BeadChips.
|
| Scan protocol |
Following washing and staining, the BeadChips were imaged using the Illumina BeadArray Reader to measure fluorescence intensity at each probe.
|
| Data processing |
BeadChip data files are analyzed with Illumina’s GenomeStudio gene expression module (V1.6.0)
|
| |
|
| Submission date |
Nov 23, 2011 |
| Last update date |
Nov 23, 2012 |
| Contact name |
Dan Wang |
| E-mail(s) |
wangdan412@gmail.com
|
| Organization name |
RPCI
|
| Street address |
Elm & Carlton Streets
|
| City |
buffalo |
| State/province |
NY |
| ZIP/Postal code |
14263 |
| Country |
USA |
| |
|
| Platform ID |
GPL6883 |
| Series (1) |
| GSE33918 |
The small molecule compound P2 in HCT116p53-/- and HCT116p53+/+ cells |
|
