|
Status |
Public on Dec 01, 2011 |
Title |
ikk-/- Reverse crosslink 6hr IFNb BMM |
Sample type |
SRA |
|
|
Source name |
bone marrow macrophages
|
Organism |
Mus musculus |
Characteristics |
genotype: Ikbke-/- cell type: Bone marrow macrophages chip antibody: none treatment: IFNβ for 6 hour genetic background: C58BL/6
|
Growth protocol |
Bone marrow from Ikbke+/+ and Ikbke-/- mice was isolated, differentiated into macrophages with macrophage colony-stimulating factor (M-CSF), and either untreated or treated with IFNb or IFNg for six hours before crosslinking for chromatin isolation.
|
Extracted molecule |
genomic DNA |
Extraction protocol |
Standard Illumina library construction
|
|
|
Library strategy |
ChIP-Seq |
Library source |
genomic |
Library selection |
ChIP |
Instrument model |
Illumina Genome Analyzer IIx |
|
|
Description |
Input control for IP
|
Data processing |
STAT1 binding sites were identified with the program MACS, using the corresponding reverse cross-link sample as a control. mm9
|
|
|
Submission date |
Nov 23, 2011 |
Last update date |
May 15, 2019 |
Contact name |
Jason Gertz |
E-mail(s) |
jgertz@hudsonalpha.com
|
Organization name |
HudsonAlpha Institute
|
Street address |
601 Genome Way
|
City |
Huntsville |
State/province |
AL |
ZIP/Postal code |
35806 |
Country |
USA |
|
|
Platform ID |
GPL11002 |
Series (1) |
GSE33913 |
IKK-epsilon regulates the balance between the type I and type II interferon responses |
|
Relations |
SRA |
SRX109340 |
BioSample |
SAMN00760844 |