|
| Status |
Public on Jan 20, 2012 |
| Title |
BrdU-IP-seq WT 1 |
| Sample type |
SRA |
| |
|
| Source name |
BrdU-IP-seq WT
|
| Organism |
Saccharomyces cerevisiae |
| Characteristics |
strain: WT
cell stage: 1hr HU arrest after release from 4hr α-factor arrest
|
| Treatment protocol |
BrdU-Incorperated-DNA was extracted as described in Viggiani et al. 2010, Cold Spring Harbor Protocols.
|
| Growth protocol |
Cells grown in YEPD at room temp with α-factor for 4 hours. Then cells were spun down and resuspended in YEPD+HU+BrdU and grown for 1hr prior to harvesting.
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Samples were prepared for sequencing using Illumina chIP-seq protocol.
|
| |
|
| Library strategy |
ChIP-Seq |
| Library source |
genomic |
| Library selection |
ChIP |
| Instrument model |
Illumina Genome Analyzer II |
| |
|
| Description |
BrdU-IP-DNA from HU arrested S. cerevisiae cells
|
| Data processing |
Reads were mapped allowing 2 mismatches to the S. cerevisiae genome (release r.64). Reads were then binned into 50 bp bins, quantile normalized across experiments and median smoothed with a 50 bp window.
|
| |
|
| Submission date |
Nov 14, 2011 |
| Last update date |
May 15, 2019 |
| Contact name |
Simon Robert Vincent Knott |
| E-mail(s) |
sknott@cshl.edu
|
| Organization name |
Cold Spring Harbor Laboratory
|
| Lab |
Hannon
|
| Street address |
1 Bungtown Rd
|
| City |
Cold Spring Harbor |
| State/province |
NY |
| ZIP/Postal code |
11724 |
| Country |
USA |
| |
|
| Platform ID |
GPL9377 |
| Series (2) |
| GSE33677 |
Forkhead transcription factors establish origin timing and long-range clustering in S. cerevisiae [Sequence Data] |
| GSE33704 |
Forkhead transcription factors establish origin timing and long-range clustering in S. cerevisiae |
|
| Relations |
| SRA |
SRX105548 |
| BioSample |
SAMN00754011 |
