|
| Status |
Public on Jun 13, 2024 |
| Title |
DN50 inoculated with Rhizobium HH103 at 24 hpi\, Repeat 1 |
| Sample type |
SRA |
| |
|
| Source name |
root
|
| Organism |
Glycine max |
| Characteristics |
tissue: root
treatment: DN50 inoculated with Rhizobium HH103 at 24 hpi\, Repeat 1
time: 24 dpi
genotype: DN50
|
| Treatment protocol |
These plants were inoculated with different strains of rhizobia, including the wild type HH103, derived mutants HH103ΩnopL and HH103ΩnodA and a mock control using 10mM MgSO4. The plants were inoculated with these rhizobia strains, and the roots were sampled,
|
| Growth protocol |
The soybean seedlings were grown in a greenhouse under a 16 h light / 8 h dark cycle at a temperature of 25 °C.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extract by Trizol
Used by VAHTS Universal V10 RNA-seq Library Prep Kit for Illumina
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Data processing |
RSEM was used to quantify gene abundances. Essentially, differential expression analysis was performed using the DESeq2. DEGs with |log2FC|≧1 and FDR≤ 0.05 were considered to be significantly different expressed genes.
Assembly: Phytozome genome ID: 508 • NCBI taxonomy ID: 3847
Supplementary files format and content: Counts for RNA-seq of DN50 and Gmrem1a inoculated with Rhizobium
|
| |
|
| Submission date |
Jun 09, 2024 |
| Last update date |
Jun 13, 2024 |
| Contact name |
Chao Ma |
| E-mail(s) |
otogishu0430@gmail.com
|
| Organization name |
Northeast Agriculutral University
|
| Department |
College of Agriculture
|
| Lab |
National Key Laboratory of Smart Farm Technologies and Systems of China
|
| Street address |
中国 哈尔滨 香坊区 长江路600号
|
| City |
Harbin |
| State/province |
Hei Longjiang |
| ZIP/Postal code |
150030 |
| Country |
China |
| |
|
| Platform ID |
GPL28801 |
| Series (1) |
| GSE269425 |
The type Ⅲ effector NopL interacts with GmREM1a and GmNFR5 to promote symbiosis in soybean |
|
| Relations |
| BioSample |
SAMN36780220 |
| SRA |
SRX21239133 |
