|
Status |
Public on Jun 13, 2024 |
Title |
DN50 inoculated with Rhizobium NopL mutant at 24 hpi\, Repeat 1 |
Sample type |
SRA |
|
|
Source name |
root
|
Organism |
Glycine max |
Characteristics |
tissue: root treatment: DN50 inoculated with Rhizobium NopL mutant at 24 hpi\, Repeat 1 time: 24 dpi genotype: DN50
|
Treatment protocol |
These plants were inoculated with different strains of rhizobia, including the wild type HH103, derived mutants HH103ΩnopL and HH103ΩnodA and a mock control using 10mM MgSO4. The plants were inoculated with these rhizobia strains, and the roots were sampled,
|
Growth protocol |
The soybean seedlings were grown in a greenhouse under a 16 h light / 8 h dark cycle at a temperature of 25 °C.
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA extract by Trizol Used by VAHTS Universal V10 RNA-seq Library Prep Kit for Illumina
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina NovaSeq 6000 |
|
|
Data processing |
RSEM was used to quantify gene abundances. Essentially, differential expression analysis was performed using the DESeq2. DEGs with |log2FC|≧1 and FDR≤ 0.05 were considered to be significantly different expressed genes. Assembly: Phytozome genome ID: 508 • NCBI taxonomy ID: 3847 Supplementary files format and content: Counts for RNA-seq of DN50 and Gmrem1a inoculated with Rhizobium
|
|
|
Submission date |
Jun 09, 2024 |
Last update date |
Jun 13, 2024 |
Contact name |
Chao Ma |
E-mail(s) |
otogishu0430@gmail.com
|
Organization name |
Northeast Agriculutral University
|
Department |
College of Agriculture
|
Lab |
National Key Laboratory of Smart Farm Technologies and Systems of China
|
Street address |
中国 哈尔滨 香坊区 长江路600号
|
City |
Harbin |
State/province |
Hei Longjiang |
ZIP/Postal code |
150030 |
Country |
China |
|
|
Platform ID |
GPL28801 |
Series (1) |
GSE269425 |
The type Ⅲ effector NopL interacts with GmREM1a and GmNFR5 to promote symbiosis in soybean |
|
Relations |
BioSample |
SAMN36780223 |
SRA |
SRX21239138 |