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Sample GSM82682 Query DataSets for GSM82682
Status Public on Sep 06, 2006
Title proband i, before exhaustive exercise
Sample type RNA
Source name white blood cells
Organism Homo sapiens
Characteristics male, healthy, no medicamentation
Extracted molecule total RNA
Extraction protocol 9ml blood were collected and erythrocyte lysis using Qiagen Erythrocyte lysis buffer was performed immediately. White blood cells were washed in icecold PBS two times. Cells were then lysed using Trizol (Invitrogen).Phase lock gel tubes (Eppendorf) were used to extract the RNA containing phase. Total RNA was extracted from the tissue lysate using an RNeasy kit (Qiagen).
Label biotin
Label protocol Approximately 3 µg of total RNA was processed to produce biotinylated cRNA targets.
Hybridization protocol standard Affymetrix procedures
Scan protocol standard Affymetrix procedures
Description proband i, before exhaustive exercise, white blood cells, male, healthy, no medicamentation
Data processing GCOS 1.2 and GeneSpring 7.1 (Agilent)
Submission date Nov 10, 2005
Last update date Sep 06, 2006
Contact name Sandy Mosig
Phone +49-3641-934813
Fax +49-3641-933950
Organization name University Hospital Jena
Department Molecular Hemostaseology - Institute of Vascular Medicine
Street address Bachstrasse 18
City Jena
ZIP/Postal code 07743
Country Germany
Platform ID GPL571
Series (1)
GSE3606 Gene expression profiles in white blood cells in response to exercise

Data table header descriptions
ID_REF Affymetrix annotation numbers
VALUE GCOS 1.2 -calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call

Data table
AFFX-BioB-5_at 341.5 P 0.000446
AFFX-BioB-M_at 510 P 0.00006
AFFX-BioB-3_at 255 P 0.000446
AFFX-BioC-5_at 901.9 P 0.000081
AFFX-BioC-3_at 841.8 P 0.000052
AFFX-BioDn-5_at 908.4 P 0.000044
AFFX-BioDn-3_at 4523 P 0.000081
AFFX-CreX-5_at 11407.8 P 0.000052
AFFX-CreX-3_at 12149.4 P 0.000044
AFFX-DapX-5_at 20.7 A 0.083574
AFFX-DapX-M_at 17.1 A 0.440646
AFFX-DapX-3_at 4.7 A 0.883887
AFFX-LysX-5_at 9.8 A 0.39692
AFFX-LysX-M_at 38.4 A 0.425962
AFFX-LysX-3_at 10.7 A 0.313723
AFFX-PheX-5_at 2.4 A 0.897835
AFFX-PheX-M_at 1.1 A 0.966323
AFFX-PheX-3_at 23.9 A 0.39692
AFFX-ThrX-5_at 6.7 A 0.699394
AFFX-ThrX-M_at 2.6 A 0.824693

Total number of rows: 22277

Table truncated, full table size 592 Kbytes.

Supplementary data files not provided

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